RT Journal Article
T1 Peptide-induced negative selection of thymocytes activates transcription of an NF-kappa B inhibitor
A1 Fiorini, Emma
A1 Reche, Pedro A
AB Negative selection eliminates thymocytes bearing autoreactive T cell receptors (TCR) via an apoptotic mechanism. We have cloned an inhibitor of NF-kappa B, I kappa BNS, which is rapidly expressed upon TCR-triggered but not dexamethasone- or gamma irradiation-stimulated thymocyte death. The predicted protein contains seven ankyrin repeats and is homologous to I kappa B family members. In class I and class II MHC-restricted TCR transgenic mice, transcription of I kappa BNS is stimulated by peptides that trigger negative selection but not by those inducing positive selection (i.e., survival) or nonselecting peptides. I kappa BNS blocks transcription from NF-kappa B reporters, alters NF-kappa B electrophoretic mobility shifts, and interacts with NF-kappa B proteins in thymic nuclear lysates following TCR stimulation. Retroviral transduction of I kappa BNS in fetal thymic organ culture enhances TCR-triggered cell death consistent with its function in selection.
YR 2002
FD 2002
LK https://hdl.handle.net/20.500.14352/58249
UL https://hdl.handle.net/20.500.14352/58249
LA eng
DS Docta Complutense
RD 10 dic 2023