%0 Journal Article
%A Fresco-Taboada, Alba
%A Serra, Immacolata
%A Arroyo Sánchez, Miguel
%A Fernández-Lucas, Jesús
%A De La Mata Riesco, Mª Isabel
%A Terreni, Marco
%T Development of an immobilized biocatalyst based on Bacilluspsychrosaccharolyticus NDT for the preparative synthesis oftrifluridine and decytabine
%D 2016
%@ 0920-5861
%U https://hdl.handle.net/20.500.14352/23393
%X The immobilization of Bacillus psychrosaccharolyticus nucleoside 2 -deoxyribosyltransferase was deeply investigated and finally optimized. The best immobilization procedure resulted to be ionic adsorption on PEI 600 Da agarose followed by crosslinking with 70% oxidized dextran (20 kDa). The percentage of recovered activity was further improved (from 21% to 33%) by the addition of 20% glycerol to the immobilization mixture. The resulting biocatalyst showed a stability profile similar to that of the soluble enzyme and it was used for the preparative synthesis oftrifluridine and decytabine obtaining conversions ranging from 50% to 76%.
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