RT Journal Article
T1 Development of an immobilized biocatalyst based on Bacilluspsychrosaccharolyticus NDT for the preparative synthesis oftrifluridine and decytabine
A1 Fresco-Taboada, Alba
A1 Serra, Immacolata
A1 Arroyo Sánchez, Miguel
A1 Fernández-Lucas, Jesús
A1 De La Mata Riesco, Mª Isabel
A1 Terreni, Marco
AB The immobilization of Bacillus psychrosaccharolyticus nucleoside 2 -deoxyribosyltransferase was deeply investigated and finally optimized. The best immobilization procedure resulted to be ionic adsorption on PEI 600 Da agarose followed by crosslinking with 70% oxidized dextran (20 kDa). The percentage of recovered activity was further improved (from 21% to 33%) by the addition of 20% glycerol to the immobilization mixture. The resulting biocatalyst showed a stability profile similar to that of the soluble enzyme and it was used for the preparative synthesis oftrifluridine and decytabine obtaining conversions ranging from 50% to 76%.
PB Elsevier
SN 0920-5861
YR 2016
FD 2016-01
LK https://hdl.handle.net/20.500.14352/23393
UL https://hdl.handle.net/20.500.14352/23393
LA eng
NO Ministerio de Ciencia e Innovación (MICINN)
NO Comunidad de Madrid
DS Docta Complutense
RD 5 abr 2025