%0 Journal Article
%A Castellanos, Elena
%A Aranaz Martín, Alicia
%A De Buck, Jeroen
%T Rapid identification and differentiation of Mycobacterium avium subspecies paratuberculosis types by use of real-time PCR and high-resolution melt analysis of the MAP1506 locus
%D 2010
%@ 1098-660X
%U https://hdl.handle.net/20.500.14352/45190
%X High-resolution melt (HRM) analysis can identify sequence polymorphisms by comparing the melting curves of amplicons generated by real-time PCR amplification. We describe the application of this technique to identify Mycobacterium avium subspecies paratuberculosis types I, II, and III. The HRM approach was based on type-specific nucleotide sequences in MAP1506, a member of the PPE (proline-proline-glutamic acid) gene family.
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