RT Journal Article
T1 Rapid identification and differentiation of Mycobacterium avium subspecies paratuberculosis types by use of real-time PCR and high-resolution melt analysis of the MAP1506 locus
A1 Castellanos, Elena
A1 Aranaz Martín, Alicia
A1 De Buck, Jeroen
AB High-resolution melt (HRM) analysis can identify sequence polymorphisms by comparing the melting curves of amplicons generated by real-time PCR amplification. We describe the application of this technique to identify Mycobacterium avium subspecies paratuberculosis types I, II, and III. The HRM approach was based on type-specific nucleotide sequences in MAP1506, a member of the PPE (proline-proline-glutamic acid) gene family.
PB American Society for Microbiology
SN 1098-660X
YR 2010
FD 2010-04
LK https://hdl.handle.net/20.500.14352/45190
UL https://hdl.handle.net/20.500.14352/45190
LA eng
NO Ministerio de Ciencia e Innovación (MICINN)
DS Docta Complutense
RD 28 abr 2024