RT Journal Article
T1 Effect of chemically modified tetracycline-8 (CMT-8) on hematology, blood chemistry, cytokines and peripheral blood lymphocyte subsets of healthy dogs
A1 Sainz Rodríguez, Ángel
A1 García-Sancho Téllez, Mercedes Guadalupe
A1 Rodríguez Franco, Fernando
A1 San Andrés Larrea, Manuel Ignacio
A1 Rodríguez Fernández, Casilda
A1 Lucas Burneo, José Julio De
A1 San Andrés Larrea, María Dolores De
A1 Agulla Pérez, Beatriz
A1 Villaescusa Fernández, Alejandra
AB Tetracyclines are antibiotics widely used in human and veterinary medicine. Effects on the immune system and inflammatory response, including effects on blood leukocytes proliferation and function and in cytokines synthesis, have been described. Chemically modified tetracyclines (CMT) have lost their antimicrobial activity, but maintain these other properties. This study analyzes the effect of chemically modified tetracycline-8 (CMT-8) on the evolution of complete blood count, blood chemistry, the mRNA expression of selected cytokines and peripheral blood lymphocyte subpopulations distribution in healthy dogs. CMT-8 at a dose of 10 mg/kg once daily was administered per os to six healthy dogs. A control group of five healthy dogs, living in the same conditions than dogs treated with CMT-8, received placebo with an identical therapeutic regimen. When given at the doses used in this study, no side effects of CMT-8 were detected, suggesting a good tolerance and a limited toxicity of the drug. Dogs treated with CMT-8 showed a gradual increase in mean corpuscular hemoglobin. The administration of CMT-8 in healthy dogs did not affect blood mRNA expression of IFN-γ, TNFα, IL-4, IL-6, IL-10, IL-12 p40 and IL-13. However, the lymphocytes expressing class II MHC on their surface decreased during the first two weeks of CMT-8 treatment and subsequently increased for the next three months. Considering the absence of antimicrobial properties of the drug, the effects of CMT-8 detected in this study seem to be unrelated to the classical antimicrobial activity attributed to tetracyclines.
PB Elsevier
SN 0034-5288
YR 2021
FD 2021
LK https://hdl.handle.net/20.500.14352/95653
UL https://hdl.handle.net/20.500.14352/95653
LA eng
NO Sainz Á, García-Sancho M, Rodríguez-Franco F, San Andrés MI, Rodríguez C, De Lucas JJ, Andrés MDS, Agulla B, Villaescusa A. Effect of chemically modified tetracycline-8 (CMT-8) on hematology, blood chemistry, cytokines and peripheral blood lymphocyte subsets of healthy dogs. Research in Veterinary Science 2021; 136: 200–208. [DOI: 10.1016/j.rvsc.2021.02.022]
NO Ministerio de Economía y Competitividad (España)
DS Docta Complutense
RD 11 abr 2025