RT Book, Section
T1 Differential scanning calorimetry of protein-lipid interactions
A1 Cañadas Benito, Olga
A1 Casals Carro, María Cristina
A2 Kleinchmidt, Jorg
AB Differential scanning calorimetry (DSC) is a highly sensitive non-perturbing technique for measuring the thermodynamic properties of thermally induced transitions. This technique is particularly useful for the characterization of lipid/protein interactions. This chapter presents an introduction to DSC instrumentation, basic theory, and methods and describes DSC applications for characterizing protein effects on model lipid membranes. Examples of the use of DSC for the evaluation of protein effects on modulation of membrane domains and membrane stability are given.
PB Springer Science+Business Media
SN 978-1-62703-274-2
YR 2012
FD 2012
LK https://hdl.handle.net/20.500.14352/100928
UL https://hdl.handle.net/20.500.14352/100928
LA eng
NO Cañadas O, Casals C. Differential scanning calorimetry of protein-lipid interactions. In Lipid-protein interactions: Methods and Protocols. Methods Mol Biol. 974:55-71. J. Kleinchmidt (ed.). 2013, doi: 10.1007/978-1-62703-275-9_4, Print ISBN: 978-1-62703-274-2. Editorial: Springer Science+Business Media, New York
NO This work was supported by the Ministerio de Ciencia e Innovacion (SAF2009-07810) and Instituto de Salud Carlos III (cibeRESCB06/06/0002).
NO Ministerio de Ciencia e Innovacion (España)
NO Instituto de Salud Carlos III
DS Docta Complutense
RD 13 ago 2024