RT Journal Article
T1 High resolution TaqMan real-time PCR approach to detect hazelnut DNA encoding for ITS rDNA in foods
A1 López-Calleja, Inés María
A1 Cruz, Silvia de la
A1 Pegels, Nicolette
A1 González Alonso, María Isabel
A1 García Lacarra, Teresa
A1 Martín De Santos, María Del Rosario
AB A broad range of foods have been described as causing allergies, but the majority of allergic reactions can be ascribed to a limited number of food components. Recent extensive surveys showed how tree nuts, particularly hazelnut (Corylus avellana L.) seeds, rank amongst the most important sources of food allergy. In order to protect the allergic consumer, efficient and reliable methods are required for the detection of allergenic ingredients. For this purpose, we have developed a real-time polymerase chain reaction (PCR) for detection of hazelnut in commercial food products. In this way a specific hazelnut primer pair based on the ITS marker (70 bp) and a nuclease (TaqMan) probe labelled with FAM and BHQ were designed. Sensibility of real-time PCR was determined by analysis of raw and heat treated hazelnut-wheat flour mixtures with a range of detection of 0.1–100,000 ppm. Practical applicability of the real-time PCR assay developed for determining hazelnut in different food matrices was investigated by analyzing 179 commercial foodstuffs comprising snacks, biscuits, chocolates, bonbons, creams, nut bars, ice creams, precooked meals, breads, beverages, yogurts, cereals, meat products, rice cake and nougat. From the total of samples analyzed, 40 commercial food products that didn’t declare hazelnut nor traces on the label were found to contain hazelnut. The real-time PCR method proposed herein due to its high sensitivity facilitates the detection of hazelnut traces in commercial food products and can also be useful for monitoring the effectiveness of cleaning processes and as consequence, can help to prevent the food allergic consumer from unintentional ingestion of hidden allergens.
PB Elsevier
SN 0308-8146
YR 2013
FD 2013
LK https://hdl.handle.net/20.500.14352/94207
UL https://hdl.handle.net/20.500.14352/94207
LA eng
NO López-Calleja, I. M., de la Cruz, S., Pegels, N., González, I., García, T., & Martín, R. (2013). High resolution TaqMan real-time PCR approach to detect hazelnut DNA encoding for ITS rDNA in foods. Food chemistry, 141(3), 1872–1880. https://doi.org/10.1016/j.foodchem.2013.05.076
NO Comunidad de Madrid
NO Ministerio de Ciencia e Innovación (España)
DS Docta Complutense
RD 15 dic 2025