RT Journal Article
T1 A role for Myosin in triggering and executing amnioserosa cell delaminations during dorsal closure
A1 Gorfinkiel Haim, Nicole
A1 Ferrer, Yanara
A1 Recalde, Jon
A1 Gutiérrez, Javier
A1 Sáez, Guillermo
AB The remodeling of epithelial tissues is a critical process in morphogenesis, often involving the apoptotic removal of individual cells while preserving tissue integrity. In Drosophila, the amnioserosa—a highly dynamic extra-embryonic tissue—undergoes extensive remodeling, culminating in its complete elimination at the end of dorsal closure. While apoptotic cell delaminations in the amnioserosa have been proposed to contribute to dorsal closure, the cellular mechanisms underlying this process remain poorly understood. In this study, we have investigated actomyosin dynamics during cell delaminations and analyzed the consequences of perturbing non-muscle Myosin activity globally in the entire tissue as well as locally in groups of cells. We found that Myosin plays an essential role in both triggering and executing cell delaminations, with high Myosin contractility promoting cell delamination via caspase activation. Additionally, our results suggest that cell delaminations are governed by both cell-autonomous Myosin dynamics and mechanical cues from the tissue environment. Together, these findings provide new insights into the regulation of epithelial cell removal and the complex interplay between apoptotic and mechanical signals during tissue remodeling.
PB Nature Research
YR 2025
FD 2025-09
LK https://hdl.handle.net/20.500.14352/125215
UL https://hdl.handle.net/20.500.14352/125215
LA eng
NO Gorfinkiel, N., Ferrer, Y., Recalde, J. et al. A role for Myosin in triggering and executing amnioserosa cell delaminations during dorsal closure. Sci Rep 15, 32160 (2025). https://doi.org/10.1038/s41598-025-16032-2
NO Ministerio de Ciencia e Innovación (España)
DS Docta Complutense
RD 20 ene 2026