RT Journal Article
T1 Colonization and biofilm formation of the extremely acidophilic archaeon Ferroplasma acidiphilum
A1 Zhang, Ruiyong
A1 Bellenberg, Sören
A1 Castro Ruiz, Laura
A1 Neu, Thomas
A1 Sand, Wolfgang
A1 Vera, Mario
AB Ferroplasma spp. are widely distributed in acid mine drainage (AMD) and biomining environments at mesophilic and moderately elevated temperatures, at low pH and high concentrations of iron and other metal ions. Microbial attachment and biofilm formation on metal sulfides are of great importance during bioleaching. In this work, several cultivation and microscopical techniques were applied to investigate the biofilm development of Ferroplasma acidiphilum. Biofilms were heterogeneously distributed on filters over time, and varied within the different growth conditions such as supplementation with glucose. Additionally, cell distribution, biofilm formation as well as EPS production of F. acidiphilum cells forming biofilms on pyrite were observed by confocal laser scanning microscopy (CLSM), scanning electron microscopy (SEM) and atomic force microscopy (AFM) combined with epifluorescence microscopy (EFM). Cells formed a monolayer biofilm and were preferably attached to the cracks/defects of pyrite surfaces. Biofilm and planktonic cells exhibited significant morphological differences. Capsular EPS were observed in both biofilm and planktonic cells.
PB Elsevier
SN 0304-386X
YR 2014
FD 2014
LK https://hdl.handle.net/20.500.14352/92303
UL https://hdl.handle.net/20.500.14352/92303
LA eng
NO Zhang, R.; Bellenberg, S.; Castro, L.; Neu, T. R.; Sand, W.; Vera, M. Colonization and biofilm formation of the extremely acidophilic archaeon Ferroplasma acidiphilum. Hydrometallurgy 2014, 150, 245-252 DOI:10.1016/j.hydromet.2014.07.001.
NO Chinese Ministry of Education
DS Docta Complutense
RD 7 oct 2024