RT Journal Article
T1 Opposite auxin dynamics determine the gametophytic and embryogenic fates of the microspore
A1 Pérez Pérez, Yolanda
A1 Solís González, María Teresa
A1 Albacete, Alfonso
A1 Testillano, Pilar S.
AB The microspore can follow two different developmental pathways. In vivo microspores follow the gametophytic program to produce pollen grains. In vitro, isolated microspores can be reprogrammed by stress treatments and follow the embryogenic program, producing doubled-haploid embryos. In the present study, we analyzed the dynamics and role of endogenous auxin in microspore development during these two different scenarios, in Brassica napus. We analyzed auxin concentration, cellular accumulation, the expression of the TAA1 auxin biosynthesis gene, and the PIN1-like efflux carrier gene, as well as the effects of inhibiting auxin biosynthesis by kynurenine on microspore embryogenesis. During the gametophytic pathway, auxin levels and TAA1 and PIN1-like expression were high at early stages, in tetrads and tapetum, while they progressively decreased during gametogenesis in both pollen and tapetum cells. In contrast, in microspore embryogenesis, TAA1 and PIN1-like genes were upregulated, and auxin concentration increased from the first embryogenic divisions. Kynurenine treatment decreased both embryogenesis induction and embryo production, indicating that auxin biosynthesis is required for microspore embryogenesis initiation and progression. The findings indicate that auxin exhibits two opposite profiles during these two microspore developmental pathways, which determine the different cell fates of the microspore.
PB MDPI
SN 1661-6596
YR 2023
FD 2023-07
LK https://hdl.handle.net/20.500.14352/121662
UL https://hdl.handle.net/20.500.14352/121662
LA eng
NO Pérez-Pérez, Y.; Solís, M.T.; Albacete, A.; Testillano, P.S. Opposite Auxin Dynamics Determine the Gametophytic and Embryogenic Fates of the Microspore. Int. J. Mol. Sci. 2023, 24, 11177. https://doi.org/10.3390/ijms241311177
NO Funding:This research was funded by Projects PID2020-113018RB-I00 (funded by MCIN/AEI/10.13039/501100011033), TED2021-129633B-I00 and CPP2021-008750 (funded by MCIN/AEI/10.13039/501100011033 and NextGenerationEU/PRTR).
NO European Commission
NO Ministerio de Ciencia e Innovación (España)
NO Agencia Estatal de Investigación (España)
DS Docta Complutense
RD 24 dic 2025