RT Journal Article T1 Production and characteristics of debaryomyces hansenii killer toxin A1 Marquina Díaz, Domingo A1 Barroso, J. A1 Santos de la Sen, Antonio A1 Martínez Peinado, José AB The optimal conditions for the production of the killer toxin of Debaryomyces hansenii CYC 1021 have been studied. The lethal activity of the killer toxin increased with the presence of NaCl in the medium used for testing the killing action. Production of the killer toxin was stimulated in the presence of proteins of complex culture media. Addition of nonionic detergents and other additives, such as dimethylsulfoxide enhanced killer toxin production significantly. Killer toxin secretion pattern followed the growth curve and reached its maximum activity at the early stationary phase. Optimal stability was observed at pH 4.5 and temperatures up to 20 °C. Above pH 4.5 a steep decrease of the stability was noted. The activity was hardly detectable at pH 5.1. PB Elsevier SN 0944-5013 YR 2001 FD 2001 LK https://hdl.handle.net/20.500.14352/108224 UL https://hdl.handle.net/20.500.14352/108224 LA eng NO Marquina, D., et al. «Production and Characteristics of Debaryomyces Hansenii Killer Toxin». Microbiological Research, vol. 156, n.o 4, 2001, pp. 387-91. DOI.org (Crossref), https://doi.org/10.1078/0944-5013-00117. DS Docta Complutense RD 21 abr 2025