RT Journal Article
T1 Histopathologic and immunohistochemical findings in the placentas and fetuses of domestic swine naturally infected with Brucella suis biovar 2
A1 Rebollada Merino, Agustín Miguel
A1 García-Seco Romero, María Teresa
A1 Pérez Sancho, Marta
A1 Domínguez Rodríguez, Lucas José
A1 Rodríguez Bertos, Antonio Manuel
AB Porcine brucellosis, which is caused by Brucella suis biovar (bv) 2, is a re-emerging disease that causes reproductive problems in pigs in Europe. The pathogenesis and lesions of B. suis intrauterine infection are poorly characterized; characterization could facilitate the diagnosis and investigation of porcine brucellosis. We collected samples of placentas and fetuses for histologic and microbiologic studies during an outbreak of abortions on a pig-breeding farm in Spain. Brucella was cultured from the vaginal swabs obtained from sows that had aborted, some placentas, and fetal tissues (spleen, liver, lung, gastric content); molecular testing confirmed B. suis bv 2 infection. Histologically, there was necrotizing and hemorrhagic placentitis; suppurative hepatitis; lymphoid depletion and sinusoidal histiocytosis in the spleen, lymph nodes, and thymus; and bronchointerstitial pneumonia. Hemorrhages were observed in the umbilical cord, heart, kidneys, and brain. We detected Brucella by immunohistochemistry (IHC) in all of the placentas and fetal organs studied, specifically in the trophoblasts of the chorionic epithelium, in the cytoplasm of macrophages in the chorionic stroma, and extracellularly in necrotic debris. Furthermore, we assessed the lymphocyte population in the placentas through the use of IHC (anti-CD3, anti- Pax5 antibodies), revealing that the lymphocytic response was composed of T cells but not B cells.
PB Sage
SN 1040-6387
SN 1943-4936
YR 2023
FD 2023-03-29
LK https://hdl.handle.net/20.500.14352/107532
UL https://hdl.handle.net/20.500.14352/107532
LA eng
NO Rebollada-Merino, A., García-Seco, T., Pérez-Sancho, M., Domínguez, L., & Rodríguez-Bertos, A. (2023). Histopathologic and immunohistochemical findings in the placentas and fetuses of domestic swine naturally infected with Brucella suis biovar 2. Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 35(3), 258–265. https://doi.org/10.1177/10406387231163867
NO Ministerio de Ciencia, Innovación y Universidades
DS Docta Complutense
RD 7 oct 2024