RT Journal Article
T1 “Inhibiting inhibitors”: Preliminary results of a new “DNA extractionamplification” disinhibition technique in critical human samples
A1 Gomes, Cláudia
A1 Alonso Conde, Alejandro
A1 Marquina Díaz, Domingo
A1 Guardià, Marc
A1 López Matayoshi, César Yoshi
A1 Palomo Díez, Sara
A1 Perea Pérez, Bernardo
A1 Gibaja, Juan F.
A1 Arroyo Pardo, Eduardo
AB The present study focused on the genetic analysis of a presumptive Bronze Age family burial, with at least six skeletons (Plana Castell, Barcelona, Spain). In order to investigate the individuals’ biological affiliation, a molecular study was undertaken, but it was impossible to obtain a positive DNA amplification. The excavation data indicated that the skeletons were buried in an old location used as a dumping ground, being very likely the presence of biological and/or chemical contaminants. To overcome this difficulty, we undertook several modifications both in the extraction as in the amplification protocol, like MgCl2concentration, or the inclusion of Bovine Serum Albumin (BSA).Despite their antiquity, the preliminary results indicate that all samples were effectively inhibited, and for the first time we were able to obtain mtDNA results, specifically, for the Hypervariable mitochondrial region 1. It also allowed us to infer that our protocol performance increased considerable, mainly the amplification modifications. In general, the best results were obtained with MgCl2 0,6 μL (20 mM) and BSA 2 mg/mL per sample. Our result is quite important in a forensic analysis, mainly in cases where corpse/s is/are buried directly in the soil.
PB Elsevier
SN 1872-4973
YR 2017
FD 2017
LK https://hdl.handle.net/20.500.14352/18440
UL https://hdl.handle.net/20.500.14352/18440
LA eng
NO Universidad Complutense de Madrid/Banco de Santander
DS Docta Complutense
RD 3 may 2024