RT Journal Article
T1 Simultaneous quantitative imaging of two PET radiotracers via the detection of positron–electron annihilation and prompt gamma emissions
A1 Pratt, Edwin C.
A1 López-Montes, Alejandro
A1 Volpe, Alessia
A1 Crowley, Michael J.
A1 Carter, Lukas M.
A1 Mittal, Vivek
A1 Pillarsetty, Nagavarakishore
A1 Ponomarev, Vladimir
A1 Udías Moinelo, José Manuel
A1 Grimm, Jan
A1 Herraiz, Joaquin L.
AB In conventional positron emission tomography (PET), only one radiotracer can be imaged at a time, because all PET isotopes produce the same two 511 keV annihilation photons. Here we describe an image reconstruction method for the simultaneous in vivo imaging of two PET tracers and thereby the independent quantification of two molecular signals. This method of multiplexed PET imaging leverages the 350–700 keV range to maximize the capture of 511 keV annihilation photons and prompt γ-ray emission in the same energy window, hence eliminating the need for energy discrimination during reconstruction or for signal separation beforehand. We used multiplexed PET to track, in mice with subcutaneous tumours, the biodistributions of intravenously injected [124I]I-trametinib and 2-deoxy-2-[18F]fluoro-D-glucose, [124I]I-trametinib and its nanoparticle carrier [89Zr]Zr-ferumoxytol, and the prostate-specific membrane antigen (PSMA) and infused PSMA-targeted chimaeric antigen receptor T cells after the systemic administration of [68Ga]Ga-PSMA-11 and [124I]I. Multiplexed PET provides more information depth, gives new uses to prompt γ-ray-emitting isotopes, reduces radiation burden by omitting the need for an additional computed-tomography scan and can be implemented on preclinical and clinical systems without any modifications in hardware or image acquisition software.
PB Nature Publishing Group
SN 2157-846X
YR 2023
FD 2023
LK https://hdl.handle.net/20.500.14352/101240
UL https://hdl.handle.net/20.500.14352/101240
LA eng
NO Pratt, E.C., Lopez-Montes, A., Volpe, A. et al. Simultaneous quantitative imaging of two PET radiotracers via the detection of positron–electron annihilation and prompt gamma emissions. Nat. Biomed. Eng 7, 1028–1039 (2023). https://doi.org/10.1038/s41551-023-01060-y
NO Ministerio de Ciencia e Innovación (España)
NO National Institutes of Health 
NO Memorial Sloan Kettering Cancer Center
DS Docta Complutense
RD 4 abr 2025