RT Journal Article
T1 Genotypic versus phenotypic characterization, with respect to susceptibility and identification, of 17 clinical isolates of Staphylococcus lugdunensis
A1 Mateo, María
A1 Maestre, Juan-Ramón
A1 Aguilar, Lorenzo
A1 Cafini, Fabio
A1 Puente, Pilar
A1 Sánchez, Paloma
A1 Alou Cervera, Luis
A1 Giménez, María José
A1 Prieto Prieto, José
AB Objectives: To compare different methods for the identification and determination of susceptibility to penicillin and methicillin of Staphylococcus lugdunensis.Methods: Seventeen clinical isolates of S. lugdunensis (identified by PCR amplification and sequencing of the rpoB gene) were studied using the ATB32-Staph, Crystal, Vitek 2 and Wider commercial systems. The clumping factor test and the tube coagulase test were also performed. Beta-lactamase production was studied by chromogenic methods. Methicillin resistance was phenotypically studied by the MRSA slide latex agglutination test, growth in MRSA agar, and the Vitek 2 and Wider systems (based on oxacillin MIC), and genotypically studied by detection of the mecA gene by PCR.Results: The clumping factor test was negative in 35.3% of strains. All isolates were correctly identified to species level by the ATB32-Staph system. Species misidentification rates were 5.9%, 23.5% and 29.4% with the Crystal, the Vitek 2 and the Wider systems, respectively, mostly as Staphylococcus haemolyticus. Beta-lactamase was present in 11.8% of strains. Whereas 76.5% and 47.1% of strains exhibited oxacillin resistance (MIC range 0.5-2 mg/L) by the Vitek 2 system and the Wider system, respectively, none of the strains was positive in the MRSA slide latex agglutination test or grew in MRSA agar. All strains lacked the mecA gene.Conclusions: The clumping factor test and some commercial systems may misidentify S. lugdunensis. Oxacillin resistance detected by commercial systems is not indicative of the presence of the mecA gene. These facts, together with beta-lactamase production, may preclude adequate treatment of infections by this virulent coagulase-negative Staphylococcus.
PB Oxford University Press
SN 1460-2091
SN 0305-7453
YR 2005
FD 2005-07-01
LK https://hdl.handle.net/20.500.14352/107039
UL https://hdl.handle.net/20.500.14352/107039
LA eng
NO Mateo M, Maestre JR, Aguilar L, Cafini F, Puente P, Sánchez P, Alou L, Giménez MJ, Prieto J. Genotypic versus phenotypic characterization, with respect to susceptibility and identification, of 17 clinical isolates of Staphylococcus lugdunensis. J Antimicrob Chemother. 2005 Aug;56(2):287-91.
DS Docta Complutense
RD 9 abr 2025