RT Journal Article
T1 Changes in cardiac energy metabolic pathways in overweighed rats fed a high-fat diet
A1 Modrego, Javier
A1 Heras Jiménez, Natalia De Las
A1 Zamorano León, José Javier
A1 Mateos Cáceres, Petra
A1 Martín Fernández, Beatriz
A1 Valero Muñoz, María
A1 Lahera Julia, Vicente
A1 López Farré, Vicente
AB Background: Heart produces ATP through long-chain fatty acids beta oxidation.Purpose: To analyze whether in ventricular myocardium, high-fat diet may modify the expression of proteins associated with energy metabolism before myocardial function was affected.Methods: Wistar Kyoto rats were divided into two groups: (a) rats fed standard diet (control; n = 6) and (b) rats fed high-fat diet (HFD; n = 6). Proteins from left ventricles were analyzed by two-dimensional electrophoresis, mass spectrometry and Western blotting.Results: Rats fed with HFD showed higher body weight, insulin, glucose, leptin and total cholesterol plasma levels as compared with those fed with standard diet. However, myocardial functional parameters were not different between them. The protein expression of 3-ketoacyl-CoA thiolase, acyl-CoA hydrolase mitochondrial precursor and enoyl-CoA hydratase, three long-chain fatty acid β-oxidation-related enzymes, and carnitine-O-palmitoyltransferase I was significantly higher in left ventricles from HFD rats. Protein expression of triosephosphate isomerase was higher in left ventricles from HFD rats than in those from control. Two α/β-enolase isotypes and glyceraldehyde-3-phosphate isomerase were significantly increased in HFD rats as compared with control. Pyruvate and lactate contents were similar in HFD and control groups. Expression of proteins associated with Krebs cycle and mitochondrial oxidative phosphorylation was higher in HFD rats.Conclusions: Expression of proteins involved in left ventricle metabolic energy was enhanced before myocardial functionality was affected in rats fed with HFD. These findings may probably indicate higher cardiac energy requirement due to weight increase by HFD.
PB Springer
SN 1436-6207
YR 2013
FD 2013-07-01
LK https://hdl.handle.net/20.500.14352/99299
UL https://hdl.handle.net/20.500.14352/99299
LA eng
NO Modrego J, de las Heras N, Zamorano-León JJ, Mateos-Cáceres PJ, Martín-Fernández B, Valero-Muñoz M, Lahera V, López-Farré AJ. Changes in cardiac energy metabolic pathways in overweighed rats fed a high-fat diet. Eur J Nutr. 2013 Mar;52(2):847-56. doi: 10.1007/s00394-012-0392-7
NO Fondo de Investigaciones de la Seguridad Social
NO Mutua Madrileña
DS Docta Complutense
RD 4 abr 2025