RT Journal Article T1 Novel gluten-free formulations from lentil flours and nutritional yeast: evaluation of extrusion effect on phytochemicals and non-nutritional factors A1 Ciudad Mulero, María A1 Fernández Ruiz, Virginia A1 Cuadrado Vives, María Carmen A1 Arribas, Claudia A1 Pedrosa, Mercedes M. A1 Berrios, José De J. A1 Pan, James A1 Morales Gómez, Patricia AB The food industry is increasingly innovating and applying new processing technologies and ingredients to develop novel food products that meet the consumers’ demand. In this study, the effect of extrusion (at 140 °C and 160 °C) was evaluated in different lentil flours formulations enriched with nutritional yeast, in terms of α-galactosides (raffinose, stachyose, verbascose), inositol phosphates (IPs), trypsin inhibitors and lectins content. The content of α-galactosides and IPs was determined by high performance liquid chromatography. Trypsin inhibitor activity (TIA) was evaluated using a small-scale quantitative assay. The lectin content was analyzed using a haemagglutination assay and a Competitive Indirect Enzyme-linked immunosorbent assay. Extrusion promoted a significant increase, up to 85% in total α-galactosides content. After extrusion, IPs content was significantly decreased and TIA as well as lectins content had a reduction higher than 90%. Extrusion demonstrated to have a beneficial effect by increasing desirable prebiotic compounds and decreasing non-nutritional factors. PB Elsevier SN 0308-8146 YR 2020 FD 2020-01-21 LK https://hdl.handle.net/20.500.14352/92838 UL https://hdl.handle.net/20.500.14352/92838 LA eng NO Ciudad-Mulero M, Fernández-Ruiz V, Cuadrado C, Arribas C, Pedrosa MM, De J. Berrios J, et al. Novel gluten-free formulations from lentil flours and nutritional yeast: Evaluation of extrusion effect on phytochemicals and non-nutritional factors. Food Chemistry 2020;315:126175. https://doi.org/10.1016/j.foodchem.2020.126175. DS Docta Complutense RD 13 abr 2025