RT Journal Article
T1 Nef-induced Alteration of the Early/Recycling Endosomal Compartment Correlates with Enhancement of HIV-1 Infectivity
A1 Rodríguez González, Ricardo
A1 Janvier, Katy
A1 Hitchin, Douglas
A1 Day, John
A1 Coleman, Scott
A1 Noviello, Colleen
A1 Bouchet, Jerome
A1 Benmerah, Alexandre
A1 Guatelli, John
A1 Benichou, Serge
AB Human immunodeficiency virus type 1 (HIV-1) Nef interacts with the clathrin-associated AP-1 and AP-3 adaptor complexes, stabilizing their association with endosomal membranes. These findings led us to hypothesize a general impact of this viral protein on the endosomal system. Here, we have shown that Nef specifically disturbs the morphology of the early/recycling compartment, inducing a redistribution of early endosomal markers and a shortening of the tubular recycling endosomal structures. Furthermore, Nef modulates the trafficking of the transferrin receptor (TfR), the prototypical recycling surface protein, indicating that it also disturbs the function of this compartment. Nef reduces the rate of recycling of TfR to the plasma membrane, causing TfR to accumulate in early endosomes and reducing its expression at the cell surface. These effects depend on the leucine-based motif of Nef, which is required for the membrane stabilization of AP-1 and AP-3 complexes. Since we show that this motif is also required for the full infectivity of HIV-1 virions, these results indicate that the positive influence of Nef on viral infectivity may be related to its general effects onearly/recycling endosomal compartments.
SN 0021-9258
YR 2005
FD 2005
LK https://hdl.handle.net/20.500.14352/98251
UL https://hdl.handle.net/20.500.14352/98251
LA eng
NO Madrid, Ricardo, et al. «Nef-Induced Alteration of the Early/Recycling Endosomal Compartment Correlates with Enhancement of HIV-1 Infectivity». Journal of Biological Chemistry, vol. 280, n.o 6, febrero de 2005, pp. 5032-44. https://doi.org/10.1074/jbc.M401202200.
DS Docta Complutense
RD 7 abr 2025