RT Journal Article
T1 CD2v Interacts with Adaptor Protein AP-1 during African Swine Fever Infection
A1 Pérez-Núñez, Daniel
A1 García-Urdiales, Eduardo
A1 Martínez-Bonet, Marta
A1 Nogal, María
A1 Barroso, Susana
A1 Revilla, Yolanda
A1 Madrid González, Ricardo
AB African swine fever virus (ASFV) CD2v protein is believed to be involved in virulence enhancement, viral hemadsorption, and pathogenesis, although the molecular mechanisms of the function of this viral protein are still not fully understood. Here we describe that CD2v localized around viral factories during ASFV infection, suggesting a role in the generation and/or dynamics of these viral structures and hence in disturbing cellular traffic. We show that CD2v targeted the regulatory trans-Golgi network (TGN) protein complex AP-1, a key element in cellular traffic. This interaction was disrupted by brefeldin A even though the location of CD2v around the viral factory remained unchanged. CD2v-AP-1 binding was independent of CD2v glycosylation and occurred on the carboxy-terminal part of CD2v, where a canonical di-Leu motif previously reported to mediate AP-1 binding in eukaryotic cells, was identified. This motif was shown to be functionally interchangeable with the di-Leu motif present in HIV-Nef protein in an AP-1 binding assay. However, we demonstrated that it was not involved either in CD2v cellular distribution or in CD2v-AP-1 binding. Taken together, these findings shed light on CD2v function during ASFV infection by identifying AP-1 as a cellular factor targeted by CD2v and hence elucidate the cellular pathways used by the virus to enhance infectivity.
PB Public Library of Science
SN 1932-6203
YR 2015
FD 2015
LK https://hdl.handle.net/20.500.14352/97923
UL https://hdl.handle.net/20.500.14352/97923
LA eng
NO Pérez-Núñez D, García-Urdiales E, Martínez-Bonet M, Nogal ML, Barroso S, Revilla Y, et al. (2015) CD2v Interacts with Adaptor Protein AP-1 during African Swine Fever Infection. PLoS ONE 10(4): e0123714. https://doi.org/10.1371/journal.pone.0123714
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO European Commission
DS Docta Complutense
RD 21 abr 2025