RT Journal Article
T1 Immobilizing Systems Biocatalysis for the Selective Oxidation of Glycerol Coupled to In Situ Cofactor Recycling and Hydrogen Peroxide Elimination
A1 Rocha Martín, Javier
A1 Acosta, Andreína
A1 Guisán, José
A1 López‐Gallego, Fernando
AB The combination of three different enzymes immobilized rationally on the same heterofunctional carrier allowed the selective oxidation of glycerol to 1,3-dihydroxyacetone (DHA) coupled to in situ redox-cofactor recycling and H2O2 elimination. In this cascade, engineered glycerol dehydrogenase with reduced product inhibition oxidized glycerol selectively to DHA with the concomitant reduction of NAD+ to NADH. NADH oxidase regenerated the NAD+ pool by oxidizing NADH to NAD+ to form H2O2 as the byproduct. Finally, catalase eliminated H2O2 to yield water and O2 as innocuous products, which avoided the spontaneous DHA oxidation triggered by H2O2. The co-immobilization of the three enzymes on the same porous carrier allowed the in situ recycling and disproportionation of the redox cofactor and H2O2, respectively, to produce up to 9.5 mM DHA, which is 18- and 6-fold higher than glycerol dehydrogenase itself and a soluble multienzyme system, respectively.
PB Wiley
SN 1867-3880
YR 2015
FD 2015
LK https://hdl.handle.net/20.500.14352/94102
UL https://hdl.handle.net/20.500.14352/94102
LA eng
NO Rocha‐Martin, Javier, et al. «Immobilizing Systems Biocatalysis for the Selective Oxidation of Glycerol Coupled to In Situ Cofactor Recycling and Hydrogen Peroxide Elimination». ChemCatChem, vol. 7, n.o 13, julio de 2015, pp. 1939-47. https://doi.org/10.1002/cctc.201500210.
NO FundingEste trabajo ha contado con el apoyo del Ministerio de Economía e Innovación de España (proyecto CTQ2009-07568). Reconocemos la biocatálisis del sistema COST action CM103. Nos gustaría agradecer a IKERBASQUE, Fundación Vasca para las Ciencias por la financiación del doctorado de FL-G. También agradecemos al gobierno de la Comunidad de Madrid por financiar la beca de doctorado para JR-M.
NO Ministerio de Economía, Industria y Competitividad (España)
NO Comunidad de Madrid
NO Fundación Vasca para las Ciencias 
DS Docta Complutense
RD 17 abr 2025