RT Journal Article
T1 Enzymatic synthesis of novel fructosylated compounds by Ffase from Schwanniomyces occidentalis in green solvents
A1 Piedrabuena, David
A1 Rumbero, Angel
A1 Pires, Elisabet
A1 Leal-Duaso, Alejandro
A1 Civera Tejuca, María Concepción
A1 Fernández-Lobato, Maria
A1 Hernáiz Gómez-Degano, María Josefa
AB The b-fructofuranosidase from the yeast Schwanniomyces occidentalis (Ffase) produces potential prebiotic fructooligosaccharides (FOS) by self-transfructosylation of sucrose, being one of the highest known producers of 6-kestose. The use of Green Solvents (GS) in biocatalysis has emerged as a sustainable alternative to conventional organic media for improving product yields and generating new molecules. In this work, the Ffase hydrolytic and transfructosylating activity was analysed using different GS, including biosolvents and ionic liquids. Among them, 11 were compatible for the net synthesis of FOS. Besides, two glycerol derivatives improved the yield of total FOS. Interestingly, polyols ethylene glycol and glycerol were found to be efficient alternative fructosyl-acceptors, both substantially decreasing the sucrose fructosylation. The main transfer product of the reaction with glycerol was a 62 g L 1 isomeric mixture of 1-O and 2-O-b-D-fructofuranosylglycerol, representing 95% of all chemicals generated by transfructosylation. Unexpectedly, the non-terminal 2-O fructo-conjugate was the major molecule catalysed during the process, while the 1-O isomer was the minor one. This fact made Ffase the first known enzyme from yeast showing this catalytic ability. Thus, novel fructosylated compounds with potential applications in food, cosmetics, and pharmaceutical fields have been obtained in this work, increasing the biotechnological interest of Ffase with innocuous GS.
PB Royal Society of Chemistry
YR 2021
FD 2021
LK https://hdl.handle.net/20.500.14352/115668
UL https://hdl.handle.net/20.500.14352/115668
LA eng
NO Piedrabuena D, Rumbero Á, Pires E, Leal-Duaso A, Civera C, Fernández-Lobato M, et al. Enzymatic synthesis of novel fructosylated compounds by Ffase fromSchwanniomyces occidentalisin green solvents. RSC Advances. 2021;11(39):24312-9.
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Ministerio de Economía y Competitividad (España)
NO Ministerio de Educación, Cultura y Deporte (España)
NO Fundación Ramón Areces
NO Gobierno de Aragón
NO European Commission
DS Docta Complutense
RD 17 abr 2025