RT Journal Article
T1 Characterization of Limnospira platensis PCC 9108 R-M and CRISPR-Cas systems
T2 Caracterizacion de Limnospira platensis PCC 9108 R-M y los sistemas CrispR-Cas
A1 Castillo López, María
A1 Guevara Acosta, Flor Govinda
A1 Baldanta Callejo, Sara
A1 Suárez Rodríguez, Patricia
A1 Agudo, Lucía
A1 Nogales, Juan
A1 Díaz Carrasco, Asunción
A1 Arribas-Aguilar, Fernando
A1 Pérez-Pérez, Julián
A1 García, Jose Luis
A1 Galán, Beatriz
A1 Navarro Llorens, Juana María
AB The filamentous cyanobacterium Limnospira platensis, formerly known as Arthrospira platensis or spirulina, is one of the most commercially important species of microalgae. Due to its high nutritional value, pharmacological and industrial applications it is extensively cultivated on a large commercial scale. Despite its widespread use, its precise manipulation is still under development due to the lack of effective genetic protocols. Genetic transformation of Limnospira has been attempted but the methods reported have not been generally reproducible in other laboratories. Knowledge of the transformation defense mechanisms is essential for understanding its physiology and for broadening their applications. With the aim to understand more about the genetic defenses of L. platensis, in this work we have identified the restriction-modification and CRISPR-Cas systems and we have cloned and characterized thirteen methylases. In parallel, we have also characterized the methylome and orphan methyltransferases using genome-wide analysis of DNA methylation patterns and RNA-seq. The identification and characterization of these enzymes will be a valuable resource to know how this strain avoids being genetically manipulated and for further genomics studies.
SN 0944-5013
YR 2023
FD 2023
LK https://hdl.handle.net/20.500.14352/92676
UL https://hdl.handle.net/20.500.14352/92676
LA eng
NO Castillo M, Guevara G, Baldanta S, Rodríguez PS, Agudo L, Nogales J, Carrasco AD, Arribas-Aguilar F, Pérez-Pérez J, García JL, Galán B, Navarro Llorens JM. Characterization of Limnospira platensis PCC 9108 R-M and CRISPR-Cas systems. Microbiol Res. 2023 Dec 9;279:127572. doi: 10.1016/j.micres.2023.127572. Epub ahead of print. PMID: 38101163.
NO Ministerio de Economía y Competitividad (España)
NO Comunidad de Madrid
NO European Commission
NO Ministerio de Ciencia e Innovación (España)
DS Docta Complutense
RD 15 ago 2024