RT Journal Article
T1 Why is less cationic lipid required to prepare lipoplexes from plasmid DNA than linear DNA in gene therapy?
A1 Muñoz Úbeda, Mónica
A1 Misra, Santosh
A1 Barrán-Berdón, Ana
A1 Aicart-Ramos, Clara
A1 Sierra, María
A1 Biswas, Joydeep
A1 Kondaiah, Paturu
A1 Junquera González, María Elena
A1 Bhattacharya, Santanu
A1 Aicart Sospedra, Emilio
AB The most important objective of the present study was to explain why cationic lipid (CL)-mediated delivery of plasmid DNA (pDNA) is better than that of linear DNA in gene therapy, a question that, until now, has remained unanswered. Herein for the first time we experimentally show that for different types of CLs, pDNA, in contrast to linear DNA, is compacted with a large amount of its counterions, yielding a lower effective negative charge. This feature has been confirmed through a number of physicochemical and biochemical investigations. This is significant for both in vitro and in vivo transfection studies. For an effective DNA transfection, the lower the amount of the CL, the lower is the cytotoxicity. The study also points out that it is absolutely necessary to consider both effective charge ratios between CL and pDNA and effective pDNA charges, which can be determined from physicochemical experiments.
PB American Chemical Society
SN 0002-7863
YR 2011
FD 2011
LK https://hdl.handle.net/20.500.14352/91143
UL https://hdl.handle.net/20.500.14352/91143
LA eng
NO Muñoz-Úbeda, Mónica, et al. «Why Is Less Cationic Lipid Required To Prepare Lipoplexes from Plasmid DNA than Linear DNA in Gene Therapy?» Journal of the American Chemical Society, vol. 133, n.o 45, noviembre de 2011, pp. 18014-17. https://doi.org/10.1021/ja204693f.
NO Ministerio de Ciencia e Innovación (España)
NO Laboratori de Llum Sincrotró (Barcelona)
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 17 abr 2025