RT Journal Article
T1 Antibacterial Effect of Functionalized Polymeric Nanoparticles on Titanium Surfaces Using an In Vitro Subgingival Biofilm Model
A1 Bueno, Jaime
A1 Virto Ruiz, Leire
A1 Toledano Osorio, Manuel
A1 Figuero Ruiz, Elena
A1 Toledano, Manuel
A1 Medina Castillo, Antonio L.
A1 Osorio, Raquel
A1 Sanz Alonso, Mariano
A1 Herrera González, David
AB This investigation aimed to evaluate the antibacterial effect of polymeric nanoparticles (NPs), functionalized with calcium, zinc, or doxycycline, using a subgingival biofilm model of six bacterial species (Streptococcus oralis, Actinomyces naeslundii, Veillonela parvula, Fusobacterium nucleatum, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans) on sandblasted, large grit, acid-etched titanium discs (TiDs). Undoped NPs (Un-NPs) or doped NPs with calcium (Ca-NPs), zinc (Zn-NPs), or doxycycline (Dox-NPs) were applied onto the TiD surfaces. Uncovered TiDs were used as negative controls. Discs were incubated under anaerobic conditions for 12, 24, 48, and 72 h. The obtained biofilm structure was studied by scanning electron microscopy (SEM) and its vitality and thickness by confocal laser scanning microscopy (CLSM). Quantitative polymerase chain reaction of samples was used to evaluate the bacterial load. Data were evaluated by analysis of variance (p < 0.05) and post hoc comparisons with Bonferroni adjustments (p < 0.01). As compared with uncovered TiDs, Dox-NPs induced higher biofilm mortality (47.21% and 85.87%, respectively) and reduced the bacterial load of the tested species, after 72 h. With SEM, scarce biofilm formation was observed in Dox-NPs TiDs. In summary, Dox-NPs on TiD reduced biofilm vitality, bacterial load, and altered biofilm formation dynamics.
PB MPDI
YR 2022
FD 2022-01-18
LK https://hdl.handle.net/20.500.14352/92445
UL https://hdl.handle.net/20.500.14352/92445
LA eng
NO Bueno, J.; Virto, L.; Toledano-Osorio, M.; Figuero, E.; Toledano, M.; Medina-Castillo, A.L.; Osorio, R.; Sanz, M.; Herrera, D. Antibacterial Effect of Functionalized Polymeric Nanoparticles on Titanium Surfaces Using an In Vitro Subgingival Biofilm Model. Polymers 2022, 14, 358. h
NO Ministry of Economy and Competitiveness
NO European Regional Development Fund (FEDER)
NO Ministry of Universities
DS Docta Complutense
RD 28 sept 2024