%0 Journal Article
%A Ruge, Christian Arnold
%A Kirch, Julian
%A Cañadas Benito, Olga
%A Schneider, Marc
%A Pérez Gil, Jesús
%A Schaefer, Ulrich Friedrich
%A Casals Carro, María Cristina
%A Lehr, Claus Michael
%T Uptake of nanoparticles by alveolar macrophages is triggered by surfactant protein A
%D 2011
%@ 1549-9634
%U https://hdl.handle.net/20.500.14352/94434
%X Understanding the bio-nano interactions in the lungs upon the inhalation of nanoparticles is a major challenge in both pulmonary nanomedicine and nanotoxicology. To investigate the effect of pulmonary surfactant protein A (SP-A) on the interaction between nanoparticles and alveolar macrophages, we used magnetite nanoparticles (110-180 nm in diameter) coated with different polymers (starch, carboxymethyldextran, chitosan, poly-maleic-oleic acid, phosphatidylcholine). Cellular binding and uptake of nanoparticles by alveolar macrophages was increased for nanoparticles treated with SP-A, whereas albumin, the prevailing protein in plasma, led to a significant decrease. A significantly different adsorption pattern of SP-A, compared to albumin was found for these five different nanomaterials. This study provides evidence that after inhalation of nanoparticles, a different protein coating and thus different biological behavior may result compared to direct administration to the bloodstream
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