RT Journal Article
T1 Uptake of nanoparticles by alveolar macrophages is triggered by surfactant protein A
A1 Ruge, Christian Arnold
A1 Kirch, Julian
A1 Cañadas Benito, Olga
A1 Schneider, Marc
A1 Pérez Gil, Jesús
A1 Schaefer, Ulrich Friedrich
A1 Casals Carro, María Cristina
A1 Lehr, Claus Michael
AB Understanding the bio-nano interactions in the lungs upon the inhalation of nanoparticles is a major challenge in both pulmonary nanomedicine and nanotoxicology. To investigate the effect of pulmonary surfactant protein A (SP-A) on the interaction between nanoparticles and alveolar macrophages, we used magnetite nanoparticles (110-180 nm in diameter) coated with different polymers (starch, carboxymethyldextran, chitosan, poly-maleic-oleic acid, phosphatidylcholine). Cellular binding and uptake of nanoparticles by alveolar macrophages was increased for nanoparticles treated with SP-A, whereas albumin, the prevailing protein in plasma, led to a significant decrease. A significantly different adsorption pattern of SP-A, compared to albumin was found for these five different nanomaterials. This study provides evidence that after inhalation of nanoparticles, a different protein coating and thus different biological behavior may result compared to direct administration to the bloodstream
PB Elsevier
SN 1549-9634
YR 2011
FD 2011
LK https://hdl.handle.net/20.500.14352/94434
UL https://hdl.handle.net/20.500.14352/94434
LA eng
NO Ruge CA, Kirch K, Cañadas O, Schneider M, Pérez-Gil J, Schaefer UF, Casals C, Lehr CM. Uptake of nanoparticles by alveolar macrophages is triggered by surfactant protein A. Nanomedicine: Nanotechnology, Biology and Medicine 2011 Dec;7:690-693
NO From the clinical editor: In this nano-toxicology study of inhaled nanoparticles, the authors investigated the effect of pulmonary surfactant protein A on the interaction between nanoparticles and alveolar macrophages utilizing magnetite nanoparticles coated with different polymers (starch, carboxymethyldextran, chitosan, poly-maleic-oleic acid, phosphatidylcholine). Cellular binding and uptake of nanoparticles increased for nanoparticles treated with SP-A, whereas albumin, the prevailing protein in plasma, led to a significant decrease.
DS Docta Complutense
RD 15 sept 2024