RT Journal Article
T1 Inter-laboratory validation of an ISO test method for measuring enzyme activities in soil samples using colorimetric substrates
A1 Cheviron, Nathalie
A1 Grondin, Virginie
A1 Marrauld, Christelle
A1 Poiroux, Françoise
A1 Bertrand, Isabelle
A1 Abadie, Josiane
A1 Pandard, Pascal
A1 Riah-Anglet, Wassila
A1 Dubois, Caroline
A1 Malý, Stanislav
A1 Marques, Catarina R.
A1 Valverde Asenjo, María Inmaculada
A1 Alonso Conde, Alejandro
A1 Marquina Díaz, Domingo
A1 Mougin, Christian
AB The evaluation of soil quality requires the use of robust methods to assess biologically based indicators. Among them, enzyme activities are used for several decades, but there is a clear need to update their measurement methods for routine use, in combining feasibility, accuracy, and reliability. To this end, the platform Biochem-Env optimized a miniaturized method to measure enzyme activities in soils using colorimetric substrates in micro-well plates. The standardization of the method was carried out within the framework of ISO/TC 190/SC 4/WG 4 “Soil quality – Biological methods” workgroup, recommending an inter-laboratory evaluation for the publication of a full ISO standard. That evaluation, managed by the platform, was based on the measurement, in six soils of contrasted physicochemical properties, of the ten soil enzyme activities described in the standard. Eight laboratories were involved in the validation study. Only 2.7% of outliers were identified from the analyses of the whole dataset. The repeatability and reproducibility of the method were determined by computing, respectively, the intra-laboratory (CVr,) and inter-laboratory (CVR) coefficients of variation for each soil and enzyme. The mean CVr ranged from 4.5% (unbuffered phosphatase) to 9.9% (α-glucosidase), illustrating a reduced variability of enzyme activities within laboratories. The mean CVR ranged from 13.8% (alkaline phosphatase) to 30.9% (unbuffered phosphatase). Despite this large CVR noticed for unbuffered phosphatase, the method was repeatable, reproducible, and sensitive. It also proved to be applicable for measuring enzyme activities in different types of soils. These results have been found successful by ISO/TC 190/SC4 and resulted in the publication of ISO 20130:2018 standard.
PB Springer
SN 0944-1344
YR 2021
FD 2021-11-25
LK https://hdl.handle.net/20.500.14352/4965
UL https://hdl.handle.net/20.500.14352/4965
LA eng
NO Cheviron, N., Grodin, V., Marrauld, C. et al. «Inter-Laboratory Validation of an ISO Test Method for Measuring Enzyme Activities in Soil Samples Using Colorimetric Substrates». Environmental Science and Pollution Research, vol. 29, n.o 20, abril de 2022, pp. 29348-57. DOI.org (Crossref), https://doi.org/10.1007/s11356-021-17173-3.
NO Agence de l’Environnement et de la Maîtrise de l’Energie
NO Fundação para a Ciência e a Tecnologia (Portugal)
NO French National Research Agency
DS Docta Complutense
RD 12 abr 2025