RT Journal Article
T1 Use of the usp45 lactococcal secretion signal sequence to drive the secretion and functional expression of enterococcal bacteriocins in Lactococcus lactis
A1 Borrero Del Pino, Juan
A1 Jiménez Martínez, Juan J.
A1 Gútiez Sainz-Pardo, Loreto
A1 Herranz Sorribes, Carmen
A1 Cintas Izarra, Luis Miguel
A1 Hernández Cruza, Pablo Elpidio
AB Replacement of the signal peptide (SP) of the bacteriocins enterocin P (EntP) and hiracin JM79 (HirJM79), produced by Enterococcus faecium P13 and Enterococcus hirae DCH5, respectively, by the signal peptide of Usp45 (SP usp45 ), the major Sec-dependent protein secreted by Lactococcus lactis, permits the production, secretion, and functional expression of EntP and HirJM79 by L. lactis. Chimeric genes encoding the SP usp45 fused to either mature EntP (entP), with or without the immunity gene (entiP) or to mature HirJM79 (hirJM79), with or without the immunity gene (hiriJM79), were cloned into the expression vector pMG36c, carrying the P32 constitutive promoter, and into pNZ8048 under control of the inducible PnisA promoter. The production of EntP and HirJM79 by most of the L. lactis recombinant strains was 1.5- to 3.7-fold higher and up to 3.6-fold higher than by the E. faecium P13 and E. hirae DCH5 control strains, respectively. However, the specific antimicrobial activity of the recombinant EntP was 1.1- to 6.2-fold higher than that produced by E. faecium P13, while that of the HirJM79 was a 40% to an 89% of that produced by E. hirae DCH5. Chimeras of SP usp45 fused to mature EntP or HirJM79 drive the production and secretion of these bacteriocins in L. lactis in the absence of specific immunity and secretion proteins. The supernatants of the recombinant L. lactis NZ9000 strains, producers of EntP, showed a much higher antimicrobial activity against Listeria spp. than that of the recombinant L. lactis NZ9000 derivatives, producers of HirJM79.
PB Springer
SN 0175-7598
SN 1432-0614
YR 2010
FD 2010-09-15
LK https://hdl.handle.net/20.500.14352/115206
UL https://hdl.handle.net/20.500.14352/115206
LA eng
NO Borrero, J., Jiménez, J.J., Gútiez, L. et al. Use of the usp45 lactococcal secretion signal sequence to drive the secretion and functional expression of enterococcal bacteriocins in Lactococcus lactis . Appl Microbiol Biotechnol 89, 131–143 (2011). https://doi.org/10.1007/s00253-010-2849-z
NO Ministerio de Educación y Ciencia (España)
NO Ministerio de Ciencia e Innovación (España)
NO Comunidad de Madrid
DS Docta Complutense
RD 6 abr 2025