RT Journal Article
T1 Evolution of human H3N2 influenza virus receptor specificity has substantially expanded the receptor-binding domain site
A1 Thompson, Andrew J.
A1 Wu, Nicholas C.
A1 Canales Mayordomo, María Ángeles
A1 Kikuchi, Chika
A1 Zhu, Xueyong
A1 Fernadez de Toro, Beatriz
A1 Cañada, Francisco J.
A1 Worth, Charli
A1 Wang, Shengyang
A1 Mcbride, Ryan
A1 Peng, Wenjie
A1 Nycholat, Corwin M.
A1 Jimenez-Barbero, Jesus
A1 Wilson, Ian A.
A1 Paulson, James C.
AB Hemagglutinins (HAs) from human influenza viruses descend from avian progenitors that bind α2–3-linked sialosides, and must adapt to glycans with α2–6-linked sialic acids on human airway cells to transmit within the human population. Since their introduction during the 1968 pandemic, H3N2 viruses have evolved over the past five decades to preferentially recognize α2–6-sialoside receptors that are elongated through addition of poly-LacNAc. Using STD-NMR, X-ray crystallography, and solid-phase glycan microarrays, we show that more recent H3N2 viruses now make increasingly complex interactions with elongated receptors, while continuously selecting for strains maintaining this phenotype. This change is accompanied by an extension of the traditional receptor binding site to include residues in key antigenic sites on the surface of HA trimers. These results help explain the propensity for selection of antigenic variants, leading to vaccine mismatching, when H3N2 viruses are propagated in chicken eggs or cells that do not contain such receptors.
PB Cell Press
SN 1934-6069
SN 1931-3128
YR 2024
FD 2024-04-29
LK https://hdl.handle.net/20.500.14352/107749
UL https://hdl.handle.net/20.500.14352/107749
LA eng
NO Thompson A J, Wu N C, Canales A, Kikuchi C,  Zhu X , Fernández de Toro B, Cañada F J, Worth C,  Wang S , McBride R , Peng W ,  Nycholat C M ,  Jiménez-Barbero J, Wilson I A, Paulson J C. Evolution of human H3N2 influenza virus receptor specificity has substantially expanded the receptor-binding domain site.  Cell Host Microbe. 2024 Feb 14; 32(2): 261–275.e4.
NO Bill and Melinda Gates Foundation, Ministerio de Ciencia e Innovación de España, Kwang Hua Educational Foundation, NIH NIAID Centers of Excellence for Influenza Research
DS Docta Complutense
RD 7 abr 2025