RT Journal Article
T1 Phenotypic and transcriptional analysis of Saccharomyces cerevisiae during wine fermentation in response to nitrogen nutrition and co-inoculation with Torulaspora delbrueckii
A1 Ruiz  Ruiz, Javier
A1 Celis Rodríguez, Miguel de
A1 Toro, María de
A1 Mendes Ferreira, Ana
A1 Rauhut, Doris
A1 Santos de la Sen, Antonio
A1 Belda Aguilar, Ignacio
AB Nitrogen content of grape musts strongly impacts on fermentation performance and wine metabolite production. As nitrogen is a limiting nutrient in most grape musts, nitrogen supplementation is a common practice that ensures yeast growth during fermentation. However, preferred nitrogen sources -as ammonium- repress the genes related to alternative nitrogen sources consumption, usually involved in aromatic compounds production. Here, we describe the effect of high ammonium doses in Saccharomyces cerevisiae fermentation performance and wine properties, and how it is affected by yeast co-inoculation in mixed (S. cerevisiae + Torulaspora delbrueckii) fermentations. In addition, an RNA-seq analysis allowed us to study the S. cerevisiae transcriptional response to ammonium nutrition and yeast interaction, demonstrating that T. delbrueckii presence affects the global S. cerevisiae transcriptional response, reducing ammonium effects at both phenotypic -fermentation kinetics and metabolite production- and transcriptional levels, under experimental conditions.
PB Elsevier
SN 0963-9969
YR 2020
FD 2020-09-18
LK https://hdl.handle.net/20.500.14352/7717
UL https://hdl.handle.net/20.500.14352/7717
LA eng
NO Ministerio de Economia, Industria y Competitividad. Centro para el Desarrollo Tecnológico Industrial (CDTI)
NO Fundación para el Conocimiento madri+d/Universidad Rey Juan Carlos
NO Universidad Complutense de Madrid
NO Hessen State Ministry of Higher Education, Research and the Arts
NO Federation of European Microbiological Societies(FEMS)
NO Agrovin S.A
NO Fundação para a Ciência e Tecnologia to Biosystems and Integrative Sciences Institute (BioISI)
DS Docta Complutense
RD 6 may 2024