RT Journal Article
T1 CC2D1B coordinates ESCRT-III activity during the mitotic reformation of the nuclear envelope
A1 Ventimiglia, Leandro N.
A1 Cuesta Geijo, Miguel Ángel
A1 Martinelli, Nicolás
A1 Caballe, Anna
A1 Macheboeuf, Pauline
A1 Miguet, Nolwenn
A1 Parnham, Ian M.
A1 Olmos Buchelt, Yolanda
A1 Carlton, Jeremy G.
A1 Weissenhorn, Winfried
A1 Martin Serrano, Juan
AB The coordinated reformation of the nuclear envelope (NE) after mitosis re-establishes the structural integrity and the functionality of the nuclear compartment. The endosomal sorting complex required for transport (ESCRT) machinery, a membrane remodeling pathway that is highly conserved in eukaryotes, has been recently involved in NE resealing by mediating the annular fusion of the nuclear membrane (NM). We show here that CC2D1B, a regulator of ESCRT polymerization, is required to re-establish the nuclear compartmentalization by coordinating endoplasmic reticulum (ER) membrane deposition around chromatin disks with ESCRT-III recruitment to the reforming NE. Accordingly, CC2D1B determines the spatiotemporal distribution of the CHMP7-ESCRT-III axis during NE reformation. Crucially, in CC2D1B-depleted cells, ESCRT activity is uncoupled from Spastin-mediated severing of spindle microtubules, resulting in persisting microtubules that compromise nuclear morphology. Therefore, we reveal CC2D1B as an essential regulatory factor that licenses the formation of ESCRT-III polymers to ensure the orderly reformation of the NE.
PB Elsevier
SN 1534-5807
YR 2018
FD 2018-12-03
LK https://hdl.handle.net/20.500.14352/105061
UL https://hdl.handle.net/20.500.14352/105061
LA eng
NO Ventimiglia LN, Cuesta-Geijo MA, Martinelli N, Caballe A, Macheboeuf P, Miguet N, et al. CC2D1B Coordinates ESCRT-III Activity during the Mitotic Reformation of the Nuclear Envelope. Developmental Cell. 2018;47(5):547-563.e6.
NO Grants and funding:WT102871MANIHR Comprehensive BRCPhD fellowship from the Région Rhône-Alpes (Cluster 10 infectiologie)Wellcome Trust Research Career Development Fellowship (093603/Z/10/Z)
NO Wellcome Trust
NO National Institute for Health and Care Research
NO Agence Nationale de la Recherche (France)
NO Institut Universitaire de France
NO Grenoble Instruct-ERIC Centre
NO French Infrastructure for Integrated Structural Biology Initiative
DS Docta Complutense
RD 6 abr 2025