RT Journal Article
T1 Characterizing the Microbial Colonization of a Dolostone Quarry: Implications for Stone Biodeterioration and Response to Biocide Treatments
A1 Cámara, Beatriz
A1 De los Ríos, Asunción
A1 Urizal, Marta
A1 Álvarez de Buergo, Mónica
A1 Varas Muriel, María Josefa
A1 Fort, Rafael
A1 Ascaso, Carmen
AB This study examines the microbial colonization of three fronts of an abandoned dolostone quarry (Redueña, Madrid, Spain) exposed to atmospheric conditions for different time periods since Roman times to the present. Through scanning electron microscopy in backscattered electron mode (SEM-BSE), endolithic colonization was predominantly detected in the most recently exposed front, while in the longer exposed quarry fronts, epilithic forms of growth were most often observed. These observations were confirmed by denaturing gradient gel electrophoresis (DGGE) analysis. Based on the distribution pattern of microbial colonization in the different quarry fronts, we then established a sequence of colonization events that took place over this long time frame. Bioalteration processes related to this sequential colonization were also identified. Characterizing these sequential processes can be useful for interpreting biodeterioration processes in historic dolostone monuments, especially those affecting constructions in the area of the Redueña stone quarry. In a second experimental stage, different biocide treatments were tested on this quarry rock to find the best way to avoid the microbial colonization effects identified. Through combined SEMBSE/ DGGE analysis, the efficacy of several biocides against the microorganisms inhabiting the dolostones was assessed after 4 and 16 months treatment. In general, all treatments were effective at reducing around 80% of the lichen cover, although effects on endolithic lithobiontic communities were dependant on how well the rock surface had been mechanically cleaned prior to treatment and gradually disappeared over time.
PB Springer
SN 0095-3628
YR 2011
FD 2011-02-26
LK https://hdl.handle.net/20.500.14352/87932
UL https://hdl.handle.net/20.500.14352/87932
LA eng
NO Received: 7 September 2010 / Accepted: 1 February 2011 / Published online: 26 February 2011© Springer Science+Business Media, LLC 2011
NO Comunidad de Madrid (España)
DS Docta Complutense
RD 6 abr 2025