RT Journal Article
T1 Dual Amperometric Immunosensor for Improving Cancer Metastasis Detection by the Simultaneous Determination of Extracellular and Soluble Circulating Fraction of Emerging Metastatic Biomarkers
A1 Barderas Manchado, Rodrigo
A1 Campuzano Ruiz, Susana
A1 Gamella Carballo, María
A1 Muñoz San Martín, Cristina
A1 Pedrero Muñoz, María
A1 Pingarrón Carrazón, José Manuel
A1 Serafín González-Carrato, Verónica
A1 Valverde De La Fuente, Alejandro
A1 Yáñez-Sedeño Orive, Paloma
A1 ben Hassine, Amira
A1 Garranzo Asensio, María
A1 Raouafi, Noureddine
AB This paper reports the development of a dual immunosensor using magnetic microcarriers (MBs) and amperometric transduction at dual screen-printed carbon electrodes (SPdCEs) for the simultaneous determination of two biomarkers: interleukin-13 receptor α2 (IL-13Rα2) and E-cadherin (E-CDH), with both extracellular and soluble fraction; oncogenic and tumor suppressor markers, respectively, of great relevance in metastatic processes. The implemented methodology involved the formation of sandwich-type immunocomplexes using specific capture antibodies immobilized onto carboxylic acid magnetic microbeads (HOOC-MBs), and biotiny-lated detector antibodies labeled with strepta-vidin horseradish peroxidase conjugates (Strep-HRP). The amperometric detection was performed by addition of hydrogen peroxide in the presence of hydroquinone (HQ) as the redox mediator. The dual immunosensing platform provided linear calibration ranges suitable for the determination of both biomarkers in liquid and solid clinical specimens as well as excellent selectivity against other cancer biomarkers. This simple handling dual bioplatform was applied to the determination of the soluble and extracellular fraction of the target biomarkers in serum and paraffined-embedded tissues from colorectal cancer (CRC) patients diagnosed at different tumor grade. The obtained results reveal great potential of this configuration to improve the reliability in diagnosing metastatic CRC.
PB Wiley
YR 2019
FD 2019-10-01
LK https://hdl.handle.net/20.500.14352/132187
UL https://hdl.handle.net/20.500.14352/132187
LA eng
NO Valverde, Alejandro, et al. «Dual Amperometric Immunosensor for Improving Cancer Metastasis Detection by the Simultaneous Determination of Extracellular and Soluble Circulating Fraction of Emerging Metastatic Biomarkers». Electroanalysis, vol. 32, n.o 4, abril de 2020, pp. 706-14. DOI.org (Crossref), https://doi.org/10.1002/elan.201900506.
NO Ministerio de Economía y Competitividad de España
NO Ministerio de Ciencia, Innovación y Universidades de España
NO Comunidad de Madrid
NO Universidad de Túnez El Manar
NO Unión Europea
DS Docta Complutense
RD 29 mar 2026