RT Journal Article
T1 ICETh1 and ICETh2, two interdependent mobile genetic elements in Thermus thermophilus transjugation
A1 Baquedano, Ignacio
A1 Mencía, Mario
A1 Blesa Esteban, Alba Mercedes
A1 Burrus, VIncent
A1 Berenguer, José
AB Cell to cell DNA transfer between Thermus thermophilus, or transjugation, requires the natural competence apparatus (NCA) of the recipient cell and a DNA donation machinery in the donor. In T. thermophilus HB27, two mobile genetic elements with functional similarities to Integrative and Conjugative Elements (ICEs) coexist, ICETh1 encoding the DNA transfer apparatus and ICETh2, encoding a putative replication module. Here, we demonstrate that excision and integration of both elements depend on a single tyrosine recombinase encoded by ICETh2, and that excision is not required but improves the transfer of these elements to a recipient cell. These findings along with previous results suggest that ICETh1 and ICETh2 depend on each other for spreading among T. thermophilus by transjugation.
SN 1462-2912
YR 2019
FD 2019-12-02
LK https://hdl.handle.net/20.500.14352/98344
UL https://hdl.handle.net/20.500.14352/98344
LA eng
NO Baquedano I, Mencía M, Blesa A, Burrus V, Berenguerfi J. ICETh1 and ICETh2, two interdependent mobile genetic elements in Thermus thermophilus transjugation. Environ Microbiol. 2020 Jan;22(1):158-169. doi: 10.1111/1462-2920.14833. Epub 2019 Dec 2. PMID: 31715642.
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Fundación Ramón Areces
NO Natural Sciences and Engineering Council of Canada (NSERC)
DS Docta Complutense
RD 6 abr 2025