RT Journal Article
T1 A simple mathematical model that describes the growth of the area and the number of total and viable cells in yeastcolonies
A1 Rivas, Eva María
A1 Gil de Prado, Elena
A1 Wrent, Petra
A1 Siloniz Jiménez, María Isabel de
A1 Correa, E.C.
A1 Conejero Meca, Francisco
A1 Murciano Cespedosa, Antonio
A1 Martínez Peinado, José
AB We propose a model, based on the Gompertz equation, to describe the growth of yeasts colonies on agar medium. This model presents several advantages: (i) one equation describes the colony growth, which previously needed twoseparate ones (linear increase of radius and of the squared radius); (ii) a similar equation can be applied to total and viable cells, colony area or colony radius, because the number of total cells in mature colonies is proportional to their area; and (iii) its parameters estimate the cell yield, the cell concentration that triggers growth limitation and the effect of this limitation on the specific growth rate. To elaborate the model, area, total and viable cells of 600 colonies of Saccharomyces cerevisiae, Debaryomyces fabryi, Zygosaccharomyces rouxii and Rhodotorula glutinis have been measured. With low inocula, viable cells showed an initial short exponential phase when colonies were not visible. This phase was shortened with higher inocula. In visible or mature colonies, cell growth displayed Gompertz-type kinetics. It was concluded that the cells growth in colonies is similar to liquid cultures only during the first hours, the rest of the time they grow, with near-zero specific growth rates, at least for 3 weeks.
PB John Wiley & Sons
SN 0266-8254, 1472-765X (online)
YR 2014
FD 2014-12
LK https://hdl.handle.net/20.500.14352/34834
UL https://hdl.handle.net/20.500.14352/34834
LA eng
NO Universidad Complutense de Madrid y Banco de Santander
DS Docta Complutense
RD 16 abr 2025