RT Journal Article
T1 Mitochondrial mitophagy protection combining rivaroxaban and aspirin in high glucose-exposed human coronary artery endothelial cell. An in vitro study
A1 Zekri, Khaoula
A1 Zamorano León, José Javier
A1 Cortina Gredilla, Mercedes
A1 López De Andrés, Ana Isabel
A1 Jiménez García, Rodrigo
A1 Navarro Cuéllar, Carlos
A1 López Farre, Antonio José
A1 Martínez Martínez, Carlos Hugo
AB Purpose: Combination of Rivaroxaban plus Aspirin improved cardiovascular outcome in patients with stable cardiovascular disease. The aim was to determine if Rivaroxaban and acetylsalicylic acid alone or in combination may protect mitochondrial mitophagy in human coronary artery endothelial cells (HCAEC) exposed to D-glucose.Methods: HCAEC were incubated under different conditions: 5 mmol/L glucose D-glucose (control), 30 mmol/L D-Glucose with and without 50 nmol/L Rivaroxaban (Rivaroxaban), 0.33 mmol/L ASA (ASA) or Rivaroxaban (12.5 nmol/L)+ASA (0.33 mmol/L; (Riva+ASA).Results: HCAEC incubated with D-glucose showed an increased Factor Xa expression. The mitochondrial content of Pink-1 and Parkin were significantly reduced in high glucose-incubated HCAEC compared to control. Rivaroxaban+ASA significantly increased the mitochondrial content of Pink-1 and Parkin, and the mitochondrial membrane potential compared to D-Glucose group. Both ASA alone and Riva+ASA reduced reactive oxygen species (ROS) and tissue factor production induced by high glucose exposure.Conclusion: Under high glucose condition combining Rivaroxaban+ASA increased the mitochondrial content of Pink-1 and Parkin, restored mitochondria membrane potential and reduced ROS and tissue factor expression in HCAEC. It suggests potential effects induced by dual use of Rivaroxaban and ASA on the coronary endothelium subjected to high glucose condition.
PB SAGE Publications
SN 1479-1641
YR 2022
FD 2022-10-16
LK https://hdl.handle.net/20.500.14352/108698
UL https://hdl.handle.net/20.500.14352/108698
LA eng
NO Zekri-Nechar K, Zamorano-León JJ, Cortina-Gredilla M, López-de-Andrés A, Jiménez-García R, Navarro-Cuellar C, López-Farré A, Martínez-Martínez CH. Mitochondrial mitophagy protection combining rivaroxaban and aspirin in high glucose-exposed human coronary artery endothelial cell. An in vitro study. Diab Vasc Dis Res. 2022 Sep-Oct;19(5):14791641221129877. doi: 10.1177/14791641221129877
DS Docta Complutense
RD 25 abr 2025