RT Journal Article
T1 Effects of aging and methionine restriction applied at old age on ROS generation and oxidative damage in rat liver mitochondria
A1 Sánchez-Román Rojas, Inés
A1 Gómez, Alexia
A1 Pérez, Irene
A1 Sánchez, Carlota
A1 Suárez, Henar
A1 Naudí, Alba
A1 Jové, Mariona
A1 López Torres, Mónica
A1 Pamplona, Reinald
A1 Barja De Quiroga Losada, Gustavo
AB It is known that a global decrease in food ingestion (dietary restriction, DR) lowers mitochondrial ROS generation (mitROS) and oxidative stress in young immature rats. This seems to be caused by the decreased methionine ingestion of DR animals. This is interesting since isocaloric methionine restriction in the diet (MetR) also increases, like DR, rodent maximum longevity. However, it is not known if old rats maintain the capacity to lower mitROS generation and oxidativestress in response to MetR similarly to young immature animals, and whether MetR implemented at old age can reverse aging-related variations in oxidative stress. In this investigation the effects of aging and 7 weeks of MetR were investigated in liver mitochondria of Wistar rats. MetR implemented at old age decreased mitROS generation, percent free radical leak at the respiratory chain and mtDNA oxidative damage without changing oxygen consumption. Protein oxidation, lipoxidation and glycoxidation increased with age, and MetR in old rats partially or totally reversed these age-related increases. Aging increased the amount of SIRT1, and MetR decreased SIRT1 and TFAM and increased complex IV. No changes were observed in the protein amounts of PGC1, Nrf2, MnSOD, AIF, complexes I, II and III, and in the extent of genomic DNA methylation. In conclusion, treating old rats with isocaloric shortterm MetR lowers mitROS production and free radical leak and oxidative damage to mtDNA, and reverses aging-related increases in protein modification. Aged rats maintain the capacity to lower mitochondrial ROS generation and oxidative stress in response to a shortterm exposure to restriction of a single dietary substance: methionine
SN 1389-5729
YR 2012
FD 2012
LK https://hdl.handle.net/20.500.14352/98077
UL https://hdl.handle.net/20.500.14352/98077
LA eng
NO Sanchez-Roman, I., Gómez, A., Pérez, I. et al. Effects of aging and methionine restriction applied at old age on ROS generation and oxidative damage in rat liver mitochondria. Biogerontology 13, 399–411 (2012). https://doi.org/10.1007/s10522-012-9384-5
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Generalitat de Catalunya
DS Docta Complutense
RD 13 abr 2025