%0 Journal Article
%A Ordóñez Gutiérrez, Lara
%A Espada Fernández, Raquel
%A Dea Ayuela, María Auxiliadora
%A Torrado Durán, Juan José
%A Bolas Fernández, Francisco
%A Alunda Rodríguez, José María
%T In vitro effect of new formulations of amphotericin B on amastigote and promastigote forms of Leishmania infantum
%D 2007
%@ 0924-8579
%U https://hdl.handle.net/20.500.14352/95903
%X The in vitro antileishmanial activities of various new amphotericin B (AMB) formulations were investigated, including microspheres of hydrophilic albumin with three AMB aggregation forms (monomeric, dimeric and multiaggregate) and the polymers of polylactic-co-glycolic acid, Resomer RG502 and RG503 with the multiaggregate AMB form. This in vitro study was performed on the extracellular promastigote form and the intracellular amastigote form of a canine strain of Leishmania infantum (UCM 20) using the infected J774 murine macrophage-like cell line. Albumin-encapsulated forms did not show any toxicity for murine cells and had lower median effective concentration (EC50) values (ca. 0.003 microg/mL) for L. infantum amastigotes than free formulations (0.03 microg/mL). In addition, the aggregation state of AMB had a notable effect on the antileishmanial activity of the drug. Results obtained in vitro point towards interest in monomeric AMB encapsulated in microspheres in the chemotherapeutic control of leishmaniasis.
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