RT Journal Article
T1 In vitro effect of new formulations of amphotericin B on amastigote and promastigote forms of Leishmania infantum
A1 Ordóñez Gutiérrez, Lara
A1 Espada Fernández, Raquel
A1 Dea Ayuela, María Auxiliadora
A1 Torrado Durán, Juan José
A1 Bolas Fernández, Francisco
A1 Alunda Rodríguez, José María
AB The in vitro antileishmanial activities of various new amphotericin B (AMB) formulations were investigated, including microspheres of hydrophilic albumin with three AMB aggregation forms (monomeric, dimeric and multiaggregate) and the polymers of polylactic-co-glycolic acid, Resomer RG502 and RG503 with the multiaggregate AMB form. This in vitro study was performed on the extracellular promastigote form and the intracellular amastigote form of a canine strain of Leishmania infantum (UCM 20) using the infected J774 murine macrophage-like cell line. Albumin-encapsulated forms did not show any toxicity for murine cells and had lower median effective concentration (EC50) values (ca. 0.003 microg/mL) for L. infantum amastigotes than free formulations (0.03 microg/mL). In addition, the aggregation state of AMB had a notable effect on the antileishmanial activity of the drug. Results obtained in vitro point towards interest in monomeric AMB encapsulated in microspheres in the chemotherapeutic control of leishmaniasis.
PB Elsevier
SN 0924-8579
YR 2007
FD 2007-10
LK https://hdl.handle.net/20.500.14352/95903
UL https://hdl.handle.net/20.500.14352/95903
LA eng
NO Ordóñez-Gutiérrez L, Espada-Fernández R, Dea-Ayuela MA, Torrado JJ, Bolás-Fernandez F, Alunda JM. In vitro effect of new formulations of amphotericin B on amastigote and promastigote forms of Leishmania infantum. Int J Antimicrob Agents. 2007 Oct;30(4):325-9. doi: 10.1016/j.ijantimicag.2007.05.013. Epub 2007 Jul 13. PMID: 17631982.
NO Ministerio de Educación y Ciencia
DS Docta Complutense
RD 6 abr 2025