RT Journal Article
T1 Cryopreservation effects on canine sperm morphometric variables and ultrastructure: comparison between vitrification and conventional freezing
A1 Cerdeira Lozano, Joaquín Vicente
A1 Sánchez Calabuig, María Jesús
A1 Pérez Gutiérrez, José Félix
A1 Hijon, M.
A1 Castaño, C.
A1 Santiago Moreno, Julián
AB Semen cryopreservation is an increasingly demanded technique in canids, particularly in order to preserve and spread high genetic value material. Sperm vitrification may represent an interesting alternative to costly and time consuming conventional freezing. The objective of this study was to evaluate the effect of sperm vitrification on sperm morphometry and ultrastructure compared to conventional freezing. Pools of nine beagle dogs were both frozen and vitrified. Computerized morphological parameters (length, wide, area and perimeter) and sperm ultrastructure, using scanning and transmission microscopy, were analysed in both fresh and in thawed/warmed samples. There were no differences (p > 0.05) between post-thaw and fresh morphometric variables of the sperm heads. However, cluster analysis revealed that sperm-heads turned out to be smaller after thawing (p < 0.05) in two of the four subpopulations. Vitrification-warming process led to an overall increase in sperm-head size. Furthermore, the sperm head size increased after warming in two subpopulations (p < 0.05). In conclusion, the variations in the sperm head area depended on the cryopreservation procedure (conventional freezing or vitrification). Conventional freezing tended to decrease the head dimensions, at least in some subpopulations, and vitrification led to an overall increase in the sperm head size. Decondensation of chromatin and plasma membrane blebbing in the head region was observed by transmission electron microscopy in several vitrified sperm, which might explain the increase of head dimensions detected by CASA-Morph system.
PB Elsevier
SN 0011-2240
YR 2020
FD 2020-08-01
LK https://hdl.handle.net/20.500.14352/107106
UL https://hdl.handle.net/20.500.14352/107106
LA eng
NO J. Cerdeira, M.J. Sánchez-Calabuig, J.F. Pérez-Gutiérrez, M. Hijon, C. Castaño, J. Santiago-Moreno, Cryopreservation effects on canine sperm morphometric variables and ultrastructure: Comparison between vitrification and conventional freezing, Cryobiology, Volume 95, 2020, Pages 164-170, https://doi.org/10.1016/j.cryobiol.2020.03.007.
NO European Commission
DS Docta Complutense
RD 17 abr 2025