RT Journal Article
T1 Alternative strategy for visceral leishmaniosis control: HisAK70-Salmonella Choleraesuis-pulsed dendritic cells
A1 Carrión Herrero, Francisco Javier
A1 Domínguez Bernal, Gustavo Ramón
A1 Martínez Rodrigo, Abel
A1 Mas Zubiri, Alicia
A1 Blanco Gutiérrez, María Del Mar
A1 Orden Gutiérrez, José Antonio
A1 Fuente López, Ricardo De La
AB Here, we describe a novel approach that exploits an attenuated mutant of Salmonella enterica serovar Choleraesuis as carrier to deliver a plasmid encoding protein HisAK70. Subsequently, dendritic cells (DCs) were pulsed with this vaccine vector. The aim of this study was to evaluate the effectiveness of the prepared HisAK70-S. Choleraesuis-pulsed DCs (HisAK70-SAL DCs) against visceral leishmaniosis (VL). In our ex vivo model of infection, the prepared formulations could decrease parasite growth by up to 80% by augmenting the production of IL-12p40 and by reducing arginase activity (ARG). Also, BALB/c mice when immunised with this formulation showed significant reduction in parasite burden in both spleen (20% of reduction) and liver (75% of reduction). The balance of the immune ratios IFN-γ/IL-10, TNF-α/IL-10, and IgG2a/IgG1 reflected the acquisition of an improved resistant phenotype in HisAK70-SAL DCs vaccinated mice compared to control mice. Our results suggest that HisAK70-SAL DCs could be a promising alternative approach for vaccine delivery that has the potential to fight Leishmania infantum (L. infantum) infection.
PB Elsevier
YR 2017
FD 2017-10
LK https://hdl.handle.net/20.500.14352/93736
UL https://hdl.handle.net/20.500.14352/93736
LA eng
NO Domínguez-Bernal, G., Martínez-Rodrigo, A., Mas, A., Blanco, M. M., Orden, J. A., De La Fuente, R., & Carrión, J. (2017). Alternative strategy for visceral leishmaniosis control: HisAK70-Salmonella Choleraesuis-pulsed dendritic cells. Comparative immunology, microbiology and infectious diseases, 54, 13–19. https://doi.org/10.1016/j.cimid.2017.07.002
NO Biodesign Institute
NO Universidad Complutense de Madrid-Santander
NO Arizona State University
NO Comunidad de Madrid
NO PLATESA (S2013/ABI-2906)
NO Ministerio de Economía y Competitividad
NO AGL2013-44100R, S2013/ABI-2906
DS Docta Complutense
RD 28 jul 2024