RT Journal Article T1 The genomic history of Iberian horses since the last Ice Age A1 Lira Garrido, Jaime A1 Tressières, Gaétan A1 Chauvey, Loreleï A1 Schiavinato, Stéphanie A1 Calvière-Tonasso, Laure A1 Seguin-Orlando, Andaine A1 Southon, John A1 Shapiro, Beth A1 Bataille, Clément A1 Pablos Fernández, Adrián AB Horses have inhabited Iberia (present-day Spain and Portugal) since the Middle Pleistocene, shaping a complex history in the region. Iberia has been proposed as a potential domestication centre and is renowned for producing world-class bloodlines. Here, we generate genome-wide sequence data from 87 ancient horse specimens (median coverage = 0.97X) from Iberia and the broader Mediterranean to reconstruct their genetic history over the last ~26,000 years. Here, we report that wild horses of the divergent IBE lineage inhabited Iberia from the Late Pleistocene, while domesticated DOM2 horses, native from the Pontic-Caspian steppes, already arrived ~1850 BCE. Admixture dating suggests breeding practices involving continued wild restocking until at least ~350 BCE, with IBE disappearing shortly after. Patterns of genetic affinity highlight the far-reaching influence of Iberian bloodlines across Europe and north Africa during the Iron Age and Antiquity, with continued impact extending thereafter, particularly during the colonization of the Americas. PB Nature Research YR 2025 FD 2025-08-02 LK https://hdl.handle.net/20.500.14352/125439 UL https://hdl.handle.net/20.500.14352/125439 LA eng NO Lira Garrido, J., Tressières, G., Chauvey, L., Schiavinato, S., Calvière-Tonasso, L., Seguin-Orlando, A., Southon, J., Shapiro, B., Bataille, C., Birgel, J., Wagner, S., Khan, N., Liu, X., Rodanés, J. M., Picazo Millán, J. V., Giralt, J., Alonso, N., Aguilera, I., Orsingher, A., … Orlando, L. (2025). The genomic history of Iberian horses since the last Ice Age. Nature Communications, 16(1), 7098. https://doi.org/10.1038/s41467-025-62266-z NO European Research Council NO CNRS/Université Paul Sabatier NO European Union’s Horizon 2020 NO Ministerio de Ciencia e Innovación NO Junta de Castilla-La Mancha NO Generalitat de Catalunya NO Gobierno de Cantabria NO MCIN/AEI/10.13039/501100011033 DS Docta Complutense RD 30 mar 2026