RT Journal Article
T1 Expression and function of a variant T cell receptor complex lacking CD3-γ
A1 Perez Aciego, Paloma
A1 Alarcon, Balbino
A1 Arnaiz Villena, Antonio
A1 Terhorst, Cox
A1 Timón, Marcos
A1 Segurado, Oscar
A1 Regueiro González-Barros, José Ramón
AB A T cell line termed DIL2 has been derived from an infant with a polyclonal T cell receptor (TCR)/CD3 cell surface expression defect. Indirect immunofluorescence showed that the expression of certain TCR/CD3 epitopes (like those detected by WT31 and BMA031 monoclonals) was strongly reduced (around five-fold) on DIL2, whereas other epitopes (like those detected by SP34 and Leu4) were only around two-fold lower than in normal T cell lines. Specific immunoprecipitates of surface-radioiodinated DIL2 cells contained TCR-alpha, TCR-beta, CD3-delta, CD3-epsilon and TCR-zeta chains, but lacked CD3-gamma. This structural TCR/CD3 variant was, however, capable of transducing certain activation signals, since normal proliferation and a low but significant calcium flux was observed in DIL2 cells after engagement with specific antibodies. Our data suggest that a functional TCR/CD3 complex can be expressed on the surface of T cells in the absence of CD3-gamma.
PB Rockefeller University Press
SN 1540-9538
YR 1991
FD 1991
LK https://hdl.handle.net/20.500.14352/107428
UL https://hdl.handle.net/20.500.14352/107428
LA eng
NO Pérez-Aciego P, Alarcón B, Arnaiz-Villena A, Terhorst C, Timón M, Segurado OG, Regueiro JR. Expression and function of a variant T cell receptor complex lacking CD3-gamma. J Exp Med. 1991 Aug 1;174(2):319-26. doi: 10.1084/jem.174.2.319. PMID: 1713248; PMCID: PMC2118903.
DS Docta Complutense
RD 9 abr 2025