RT Journal Article
T1 Thyroid hormone action in the adult brain: Gene expression profiling of the effects of single and multiple doses of triiodo-l-thyronine in the rat striatum
A1 Diez, Diego
A1 Grijota Martínez, María Carmen
A1 Agretti, Patrizia
A1 De Marco, Giuseppina
A1 Tonacchera, Massimo
A1 Pinchera, Aldo
A1 Morreale de Escobar, Gabriella
A1 Bernal, Juan
A1 Morte, Beatriz
AB Thyroid hormones have profound effects on mood and behavior, but the molecular basis of thyroid hormone action in the adult brain is relatively unknown. In particular, few thyroid hormone-dependent genes have been identified in the adult brain despite extensive work carried out on the developing brain. In this work we performed global analysis of gene expression in the adult rat striatum in search for genomic changes taking place after administration of T3 to hypothyroid rats. The hormone was administered in two different schedules: 1) a single, large dose of 25 μg per 100 g body weight (SD) or 2) 1.5 μg per 100 g body weight once daily for 5 d (RD). Twenty-four hours after the single or last of multiple doses, gene expression in the striatum was analyzed using Codelink microarrays. SD caused up-regulation of 149 genes and down-regulation of 88 genes. RD caused up-regulation of 18 genes and down-regulation of one gene. The results were confirmed by hybridization to Affymetrix microarrays and by TaqMan PCR. Among the genes identified are genes involved in circadian regulation and the regulation of signaling pathways in the striatum. These results suggest that thyroid hormone is involved in regulation of striatal physiology at multiple control points. In addition, they may explain the beneficial effects of large doses of thyroid hormone in bipolar disorders.
PB Oxford University Press
SN 0013-7227
YR 2008
FD 2008
LK https://hdl.handle.net/20.500.14352/92125
UL https://hdl.handle.net/20.500.14352/92125
LA eng
NO Diez, Diego, et al. «Thyroid Hormone Action in the Adult Brain: Gene Expression Profiling of the Effects of Single and Multiple Doses of Triiodo-l-Thyronine in the Rat Striatum». Endocrinology, vol. 149, n.o 8, agosto de 2008, pp. 3989-4000. https://doi.org/10.1210/en.2008-0350.
NO Ministerio de Educación y Ciencia (España)
NO European Commission
NO Instituto de Salud Carlos III
DS Docta Complutense
RD 6 abr 2025