RT Journal Article
T1 Watermelon Profilin: Characterization of a Major Allergen as a Model for Plant-Derived Food Profilins
A1 Cases, Bárbara
A1 Pastor Vargas, Carlos
A1 Gil Dones, Félix
A1 Perez-Gordo, Marina
A1 Maroto, Aroa
A1 las Heras, Manuel de las
A1 Vivanco Martínez, Fernando
A1 Cuesta-Herranz, Javier
AB Background: Plant profilins have been reported as minor allergens. They are a well-known pan-allergen family responsible for cross-reactivity between plant-derived foods and pollens. Watermelon profilin has been reported to be a major allergen in watermelon (Citrullus lanatus).The aim of this study was to characterize recombinant watermelon profilin, confirming its reactivity for diagnostic purposes and the development of immunotherapy.Methods: Native profilin was purified from watermelon extract by affinity chromatography using poly-L-proline. Recombinant His-tagged profilin was produced in Pichia pastoris yeast using pPICZαA vector and purified by metal chelate affinity chromatography. ELISA and immunoblot were carried out with sera from 17 watermelon-allergic patients. Biological activity was tested by the basophil activation test.Results: Native profilin and recombinant profilin were purified and identified by mass spectrometry. Both show similar IgE reactivity in vitro and are biologically active.Conclusions: Similarities were found in the IgE-binding patterns and biological activity of recombinant profilin and native profilin. Recombinant profilin may be a powerful tool for specific diagnosis.
PB Karger
SN 1018-2438
YR 2010
FD 2010
LK https://hdl.handle.net/20.500.14352/92747
UL https://hdl.handle.net/20.500.14352/92747
LA eng
NO Cases B, Pastor-Vargas C, Dones FG, Perez-Gordo M, Maroto AS, de las Heras M, Vivanco F, Cuesta-Herranz J. Watermelon profilin: characterization of a major allergen as a model for plant-derived food profilins. Int Arch Allergy Immunol. 2010;153(3):215-22. doi: 10.1159/000314361. Epub 2010 May 18. PMID: 20484919.
NO Red de Investigación de Reacciones Adversas a Alergenos y Fármacos (España)
NO Mutua Madrileña
DS Docta Complutense
RD 6 oct 2024