RT Journal Article
T1 In-depth proteomics characterization of ΔNp73 effectors identifies key proteins with diagnostic potential implicated in lymphangiogenesis, vasculogenesis and metastasis in colorectal cancer
A1 Garranzo Asensio, María
A1 Rodríguez Cobos, Javier
A1 San Millán, Coral
A1 Poves, Carmen
A1 Fernández Aceñero, María Jesús
A1 Pastor Morate, Daniel
A1 Viñal, David
A1 Montero Calle, Ana
A1 Solís Fernández, Guillermo
A1 Ceron, María Ángeles
A1 Gámez Chiachio, Manuel
A1 Rodríguez Martín, Nuria
A1 Guzmán Aránguez, Ana Isabel
A1 Barderas Manchado, Rodrigo
A1 Domínguez, Gemma
AB Colorectal cancer (CRC) is the third most common cancer and the second leading cause of cancer-related death worldwide. Alterations in proteins of the p53-family are a common event in CRC. ΔNp73, a p53-family member, shows oncogenic properties and its effectors are largely unknown. We performed an in-depth proteomics characterization of transcriptional control by ∆Np73 of the secretome of human colon cancer cells and validated its clinical potential. The secretome was analyzed using high-density antibody microarrays and stable isotopic metabolic labeling. Validation was performed by semiquantitative PCR, ELISA, dot-blot and western blot analysis. Evaluation of selected effectors was carried out using 60 plasma samples from CRC patients, individuals carrying premalignant colorectal lesions and colonoscopy-negative controls. In total, 51 dysregulated proteins were observed showing at least 1.5-foldchange in expression. We found an important association between the overexpression of ∆Np73 and effectors related to lymphangiogenesis, vasculogenesis and metastasis, such as brain-derived neurotrophic factor (BDNF) and the putative aminoacyl tRNA synthase complex-interacting multifunctional protein 1 (EMAP-II)-vascular endothelial growth factor C-vascular endothelial growth factor receptor 3 axis. We further demonstrated the usefulness of BDNF as a potential CRC biomarker able to discriminate between CRC patients and premalignant individuals from controls with high sensitivity and specificity.
PB PubMed
YR 2022
FD 2022-07-16
LK https://hdl.handle.net/20.500.14352/129894
UL https://hdl.handle.net/20.500.14352/129894
LA eng
NO Garranzo-Asensio M, Rodríguez-Cobos J, San Millán C, Poves C, Fernández-Aceñero MJ, Pastor-Morate D, Viñal D, Montero-Calle A, Solís-Fernández G, Ceron MÁ, Gámez-Chiachio M, Rodríguez N, Guzmán-Aránguez A, Barderas R, Domínguez G. In-depth proteomics characterization of ∆Np73 effectors identifies key proteins with diagnostic potential implicated in lymphangiogenesis, vasculogenesis and metastasis in colorectal cancer. Mol Oncol. 2022 Jul;16(14):2672-2692. doi: 10.1002/1878-0261.13228. Epub 2022 Jun 7. PMID: 35586989; PMCID: PMC9298678.
DS Docta Complutense
RD 21 ene 2026