RT Journal Article T1 Evolution of the activity of UGT1A1 throughout the development and adult life in a rat A1 Bustamante, Nelia A1 Cantarino Aragón, María Helena A1 Arahuetes Portero, Rosa María A1 Cubero Palero, Francisco Javier A1 Ortiz, Agustín AB Biliary excretion is the main route of disposal of bilirubin and impaired excretion results in jaundice, a well recognisable symptom of liver disease. Conjugation of bilirubin in the liver is essential for its clearance. The glucuronidation of bilirubin is catalysed by the microsomal UDP-glucuronosyltransferase UGT1A1. Patients with Crigler-Najjar syndrome type 1 and Gunn rats, mutant strain of the Wistar rats, bear an autosomal recessive disorder resulting in hyperbilirubinemia. The aim of this work is to add new data about activity of UGT1A1 during the perinatal period and adult life. The results showed that activity of UGT1A1 is detectable from day 22 of the gestation. After birth, activity of UGT1A1 gradually increases and reaches the levels of adult life. Furthermore, bilirubin azopigments have been separated and characterized by thin layer chromatography. We have found that concentration of samples by evaporation and ulterior storing at -20 degrees C seemed to be suitable for the maintenance of samples. PB Elsevier SN 0024-3205 YR 2005 FD 2005 LK https://hdl.handle.net/20.500.14352/97158 UL https://hdl.handle.net/20.500.14352/97158 LA eng NO Bustamante N, Cantarino MH, Arahuetes RM, Cubero FJ, Ortiz A. Evolution of the activity of UGT1A1 throughout the development and adult life in a rat. Life Sci. 2006 Mar 6;78(15):1688-95. doi: 10.1016/j.lfs.2005.08.018. Epub 2005 Nov 28. PMID: 16310220. NO Ministerio de Ciencia e Innovación (España) DS Docta Complutense RD 21 abr 2025