RT Journal Article
T1 Lactic Microbiota and Metabolites in Raw Cow’s Milk: Implications for Consumer Health
A1 Sánchez Beltrán, María Del Carmen
A1 Soria, Eduardo
A1 Llama Palacios, María Arantxazu
A1 Almirón, Franco
A1 Valdés Alonso, Alberto
A1 Cifuentes, Alejandro
A1 Hernández, Marta
A1 Ciudad Cabañas, María José
A1 Collado Yurrita, Luis Rodolfo
AB This study explores the microbiota and metabolite profile of raw cow’s milk, analyzing the correlation between them and their potential impact on consumer health, focusing on factors like cow’s feeding regimen (conventional vs. grazing), the herd’s geographical location (coastal vs. inland), and the thermal stability of raw milk samples. To achieve this, raw milk quality was assessed through mesophilic bacterial count and thermal stability using the 70° and 80° ethanol test. Metataxonomic analysis was performed using Illumina 16S rRNA hypervariable region sequencing, and untargeted metabolomics was conducted using UHPLC-Q/TOF MS/MS, followed by multivariate correlation analysis. All samples met quality standards. A total of 1258 prokaryotic ASVs were identified, spanning 48 bacterial phyla and one archaeal phylum, with 909 genera and 349 unidentified ASVs. Additionally, 70 metabolites were identified, including essential amino acids, vitamins, and bioactive compounds with antimicrobial, anti-inflammatory, and prebiotic properties. Significant correlations between bacterial diversity and metabolite profiles were observed. Feeding regimen and geographical location influence microbial composition and metabolite abundance, while thermal stability was linked to specific metabolites like oleamide and pyridoxal. These findings suggest that microbiota-derived metabolites can enhance the nutritional and functional value of milk. Further research is needed to understand how environmental and processing factors influence these dynamics.
PB MDPI
SN 2624-862X
YR 2025
FD 2025-05-22
LK https://hdl.handle.net/20.500.14352/125515
UL https://hdl.handle.net/20.500.14352/125515
LA eng
NO Sánchez, M.C.; Soria, E.; Llama-Palacios, A.; Almirón, F.; Valdés, A.; Cifuentes, A.; Hernández, M.; Ciudad, M.J.; Collado, L. Lactic Microbiota and Metabolites in Raw Cow’s Milk: Implications for Consumer Health. Dairy 2025, 6, 24. https://doi.org/10.3390/ dairy6030024
NO Central Lechera Asturiana
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 25 feb 2026