RT Journal Article
T1 Retinal Thickness Changes Over Time in a Murine AD Model APPNL-F/NL-F
A1 García Martín, Elena Salobrar
A1 López Cuenca, Inés
A1 Sánchez Puebla, Lídia
A1 Hoz Montañana, María Rosa de
A1 Fernández Arrabal, José A.
A1 Ramírez Sebastián, Ana Isabel
A1 Bravo Ferrer, Isbel
A1 Medina Alonso, Violeta
A1 Moro Sánchez, María Ángeles
A1 Saido, Takaomi C.
A1 Saito, Takashi
A1 Salazar Corral, Juan José
A1 Ramirez Sebastian, Jose Manuel
AB Background: Alzheimer’s disease (AD) may present retinal changes before brain pathology, suggesting the retina as an accessible biomarker of AD. The present work is a diachronic study using spectral domain optical coherence tomography (SD-OCT) to determine the total retinal thickness and retinal nerve fiber layer (RNFL) thickness in an APPNL−F/NL−F mouse model of AD at 6, 9, 12, 15, 17, and 20 months old compared to wild type (WT) animals.Methods: Total retinal thickness and RNFL thickness were determined. The mean total retinal thickness was analyzed following the Early Treatment Diabetic Retinopathy Study sectors. RNFL was measured in six sectors of axonal ring scans around the optic nerve.Results: In the APPNL−F/NL−F group compared to WT animals, the total retinal thickness changes observed were the following: (i) At 6-months-old, a significant thinning in the outer temporal sector was observed; (ii) at 15-months-old a significant thinning in the inner temporal and in the inner and outer inferior retinal sectors was noticed; (iii) at 17-months-old, a significant thickening in the inferior and nasal sectors was found in both inner and outer rings; and (iv) at 20-months-old, a significant thinning in the inner ring of nasal, temporal, and inferior retina and in the outer ring of superior and temporal retina was seen. In RNFL thickness, there was significant thinning in the global analysis and in nasal and inner-temporal sectors at 6 months old. Thinning was also found in the supero-temporal and nasal sectors and global value at 20 months old.Conclusions: In the APPNL−F/NL−F AD model, the retinal thickness showed thinning, possibly produced by neurodegeneration alternating with thickening caused by deposits and neuroinflammation in some areas of the retina. These changes over time are similar to those observed in the human retina and could be a biomarker for AD. The APPNL−F/NL−F AD model may help us better understand the different retinal changes during the progression of AD.Keywords: Alzheimer, retina, OCT, mouse model of AD, APPNL-F/NL-F
PB Frontiers Media
SN 1663-4365
YR 2021
FD 2021-01-15
LK https://hdl.handle.net/20.500.14352/7737
UL https://hdl.handle.net/20.500.14352/7737
LA eng
NO Received: 03 November 2020; Accepted: 15 December 2020;Published: 15 January 2021.
NO Instituto de Salud Carlos III
NO Ministerio de Ciencia e Innovación (MICCIN)
NO Leducq Fundation for Cardiovascular Research
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 8 may 2024